Catalog excerpts
X-GAL Agar(For the test of Coliform group) Code Directions Suspend 44.3 g of the dehydrated medium in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 °C for 15 minutes. Distribute 20 mL amounts of medium into Petri dish. For the mixing and dilution culture of liquid specimen, the medium may be used in "pour plate" technique. Remarks The medium is used for the test for coliform group in foods and drinks. Coliform group develop a blue to blue-green colony after incubation at 35 - 37 °C for 20 ± 2 hours. Growths of almost all other bacteria are inhibited, and they develop only white colony even if they grow. X-GAL (colorimetric enzyme substrate) in the medium is decomposed to bring out blue/blue-green color by B-galactosidase produced by coliform group. HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd
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