HORIZONTAL GEL ELECTROPHORESIS UNIT GEL SIZE: 5CM X 7CM 9861 Kapp Court P Box 219 Peosta, IA 52068 .O. Tele: (563) 690-0484 Toll Free: (800) 253-4942 Fax: (563) 690-0490 E-Mail: [email protected] WWW.IBISCI.COM

SAFETY INFORMATION PACKAGE CONTENTS PRODUCT SPECIFICATIONS OPERATING INSTRUCTIONS PREPARATION OF THE AGAROSE GEL - DNA PREPARATION OF THE AGAROSE GEL - RNA CASTING THE GEL REMOVING THE COMB LOADING THE SAMPLES INTO THE GEL ELECTRICAL CONNECTIONS TO THE SAFETY LID SAMPLE ELECTROPHORESIS CHOICE OF BUFFER VOLTAGE STAINING SOLUTION E. MAINTENANCE OF UNIT F. REPLACEMENT PARTS & ACCESSORIES QSH-LAB PAL ACCESSORY ITEMS AND REPLACEMENT PARTS QSH-LAB PAL COMBS G. RELATED IBI PRODUCTS H. RELATED IBI CERTIFIED REAGENTS I. REFERENCES J. LIMITED WARRANTY

A. SAFETY INFORMATION Important Safety Information! Please read this manual carefully before operating your new IBI QSH-Lab Pal unit. This manual contains important operating and safety information. To best use the product, please read the entire manual carefully prior to use. To avoid possible injury, this product should only be used for its intended purpose. B. PACKAGE CONTENTS Upon receiving this product, please verify all of the noted parts and accessories are contained in this package. Model QSH Buffer Tank Patented QSH Vented Lid Dual Gel Casting Tray One Set of 1.5mm x 5 Tooth Combs (2...

D. OPERATING INSTRUCTIONS Your new QSH-Lab Pal Horizontal Unit is cleaned and wiped prior to packaging; however, components should be washed in warm soapy water prior to use in the laboratory. A mild dish washing liquid, like Joy, works well. Gently wash the tank, lid, dual gel casting tray, and glass slides in warm soapy water, taking care not to scratch any of the acrylic components such as the tank and UVT tray. Do NOT wash Power Cords. NOTE: It is also recommended that the glass slides be cleaned with alcohol prior to use. Be certain the entire unit is dry prior to use. PREPARATION OF THE...

4.) Add ethidium bromide (IB40075) to the diluted electrophoresis buffer to a final concentration of 0.5μg/ml. NOTE: The addition of ethidium bromide to both the gel and the running buffer will result in maximum detection levels by providing high levels of sample fluorescence with an evenly low level of background. 5.) Add 6.6ml of the 1X electrophoresis buffer containing ethidium bromide made in step 4 per millimeter of gel thickness desired, up to a maximum to 50ml, to the flask containing the agarose (IB70035-40-42-45). A 50ml gel solution will make a 7.6mm thick gel. Thinner gels may be made,...

The following protocol will make 50ml of a 1.5% agarose gel containing 1X MOPS [3-(NMorpholino)-Propanesulfonic Acid]-Acetate-EDTA (MAE) buffer and 2.2M formaldehyde, resulting in a 7.5mm thick gel: 1.) Weigh 0.5gm of agarose, and place into a 125ml flask. 2.) Add 43.5ml of DEPC (or acetic anhydride) treated water. 3.) Make note of the total solution volume so that degree of evaporation can be determined and corrected for. 4.) Heat the agarose slurry in a microwave oven for 60 seconds. Swirl the flask to make sure any grains sticking to the walls enter into the solution. Undissolved agarose appears...

REMOVING THE COMB 1.) When the gel is solidified and fully opaque, carefully remove the comb with a gentle wiggling, upward motion. If the comb is difficult to remove or if a low percentage gel is being used, overlay the comb area with a small volume of 1X electrophoresis buffer to preserve the integrity of the wells. Check the wells to ensure their bases are intact. Lift the slide(s) from the casting tray by pushing up on the slide through the finger hole opening at the bottom of the casting tray (see Photo 3). CAUTION: Prolonged exposure of the Delrin combs to gels containing formaldehyde will...

ELECTRICAL CONNECTIONS TO THE SAFETY LID CAUTION: This unit is intended to be used with a power supply which detects a no current condition and prevents a current flow unless there is a completed circuit path. Use of other power supplies may compromise the safety of this unit. The QSH-Lab Pal can only be operated with the safety lid in place. Electrical current is supplied through the banana plugs to the lid which, in turn, connect to the gold plated tank connectors. A simple gravity connector in the cover ensures a complete current path, yet allows the lid to be removed from the unit without...

2.) Follow the sample migration into the gel using the loading dye as an indicator. (See “Choice of Buffer” for the Sample Loading Buffer recipe) Allow the samples to migrate until the fragments have separated, normally until the bromophenol blue dye front has migrated 3/4 of the way down the gel. NOTE: If the gel contains ethidium bromide, the progress of electrophoresis may be monitored during the run by turning off the power supply, removing the lid, and shining a mediumwave UV light onto the gel. The resolved bands will appear as orange bands against a dark purple background. DETECTION AND...

TRIS BORATE EDTA BUFFER (TBE) - IB70150: 10X Stock Solution: 1X Working Concentration: 89 mM Tris Base 108g Tris Base 89 mM Boric Acid 55g Boric Acid 2.0 mM EDTA 6.72g EDTA or 40ml 0.5M EDTA (pH 8.0) pH 8.0 H2O to 1 liter MOPS ACETATE EDTA (MAE) - IB70175: Solutions containing MOPS should be wrapped in aluminum foil and stored at room temperature. The buffer tends to yellow with age. Light yellow buffer may be used, however, dark yellow solutions should be discarded. 1X Working Concentration: 20 mM MOPS (pH 7.0) 8 mM NaOAc 1 mM EDTA (pH 8.0) 10X Stock Solution: 41.8g MOPS 800 ml DEPC treated...

E. MAINTENANCE OF UNIT Care must be observed in the handling of this unit. DO NOT expose the unit to temperatures above 60°C DO NOT expose the unit to organic solvents DO NOT clean the unit with abrasive cleaners or cleaning aids. Use mild cleaning solution (dish soap recommended) for routine cleaning. For heavier dirt, hand wash with soft cloth. In most cases, a rinse in deionized water is sufficient to clean the unit. To remove residual Ethidium Bromide from the gel unit, soak occasionally in 1% commercial bleach solution for 16 hours, and rinse well. NOTE: The degradation of acrylic by solvents...