Preparing Samples for Use on the IDEXX SNAPshot Dx* Analyzer n Step 1: Verify Sample and SNAP* Device Requirements Test Type Anticoagulated Whole Blood SNAP* Cortisol Components Requiring Room Temperature Warm-up Warm-up Time Required Pipette Used Gray or Blue (depending on protocol) SNAP* FIV/FeLV Combo Clear (provided) Clear (provided) Clear (provided) Clear (provided) Clear (provided) Clear (provided) SNAP* Feline proBNP EDTA-based plasma n Step 2: Collect and Prepare the Sample Anticoagulated Whole Blood 1. Use the appropriate sample collection device. 1. Use the appropriate serum separator tube. 2. Use the appropriate sample collection device. 1. When the sample drawer is ready, remove the Catalyst* whole blood separator from the sample drawer. 2. Draw the sample gently and transfer it to a treated sample tube. 3. Draw the sample gently and transfer it to a serum tube.‡ 4. Let the sample clot for a minimum of 20 minutes. 2. Aspirate the plasma from the whole blood separator. 1. Use the appropriate lithium heparin tube. 2. Use the appropriate sample collection device. 3. Draw the sample gently and transfer it to a lithium heparin tube.‡ 4. Gently invert the sample for 30 seconds to mix. When using an evacuated tube, such as a BD Vacutainer* tube, allow the sample to draw naturally into the tube by vacuum. When using an evacuated tube, such as a BD Vacutainer* tube, allow the sample to draw naturally into the tube by vacuum. 5. Centrifuge the sample for at least 120 seconds at a minimum of 12,000 RCF. Refer to your centrifuge operator’s manual for centrifugation settings and times. 5. Centrifuge the sample for at least 120 seconds at a minimum of 12,000 RCF. Refer to your centrifuge operator’s manual for centrifugation settings and times. n Step 3: Prepare the SNAP* Device IDEXX Customer and Technical Support U.S./Canada 1-800-248-2483 Europe idexx.eu 1. Following the on-screen instructions on the SNAPshot Dx Analyzer, dispense sample into disposable sample tube. Ensure you use the correct pipette (as indicated on the screen). 2. Dispense the appropriate amount of conjugate into the same sample tube used in step 1. 3. Gently invert the sample tube 4–5 times to mix. 4. Cortisol ONLY: Incubate the sample tube for 5 minutes. activation circle 5. Pour entire contents of sample tube into the sample well of a SNAP* device. 6. When color first appears in the activation circle, press the activator. 7. Immediately load the SNAP device into an available port on the analyzer. © 2022 IDEXX Laboratories, Inc. All rights reserved. • 06-18022-09 *Vacutainer is a registered trademark of Becton, Dickinson and Company. SNAPshot Dx, SNAP, SNAP cPL, SNAP fPL, Catalyst and 4Dx are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliate