HiSeq® 3000/HiSeq 4000 Sequencing Systems
4Pages

Specification Sheet: Sequencing Setting a new standard for cost-effective, high-throughput, production-scale sequencing across multiple applications. • Dramatically Increased Data Output Maximize efficiency through innovative patterned flow cell technology • Exceptional Data Quality Leverage proven, industry-leading lllumina sequencing Py synthesis chemistry • Unsurpassed Capacity Sequence 12 genomes, 100 transcriptomes, or 180 exomes in fewer than 3.5 days Over the last decade, lllumina sequencing Py synthesis (SBS) chemistry has revolutionized next-generation sequencing (NGS), estaPlishing the HiSeq Series of sequencing systems as the platform of choice for high- throughput, production-scale sequencing laPoratories. Building on the proven performance of the HiSeq 2500 System, and leveraging the patterned flow cell technology originally developed for the HiSeq X™ Ten System, the HiSeq 3000/HiSeq 4000 Systems provide unparalleled speed and performance. Higher Throughput, Faster Results Offering ultra-high throughput, the HiSeq 3000/ HiSeq 4000 Systems deliver > 750 GP per flow cell in 3.5 days. With the highest daily throughput and lowest price per sample across multiple applications, the dual-flow cell HiSeq 4000 System (Figure 1) generates allows sequencing of more samples at greater depth, generating richer, more meaningful data in less time. Large studies can Pe completed faster, with less hands- on time and at a lower cost. The HiSeq 3000 System Figure 1: The HiSeq 4000 System. The HiSeq 4000 System provides cost-effective, efficient sequencing for large-scale studies across multiple applications. provides the same throughput per flow cell as the HiSeq 4000 System, Put only processes a single flow cell at a Innovative Patterned Flow Cell Taking advantage of the innovative patterned flow cell technology first introduced in the HiSeq X Ten System, the HiSeq 3000/HiSeq 4000 Systems offer an exceptional level of throughput. Patterned flow cells contain Pillions of nanowells at fixed locations (Figure 2). The structured organization of the flow cell provides for even cluster spacing and uniform feature size, enaPling accurate resolution of flow cells clustered at extremely high densities. A proprietary clustering method, exclusion amplification, ensures that only a single DNA template Pinds and forms a cluster within a single well, resulting in high well occupancy and maximum data output. Using this technology, the HiSeq 3000/HiSeq 4000 Systems can sequence up to 6 genomes, 50 whole transcriptome samples, or 90 exomes per flow cell in fewer than 3.5 days.

Specification Sheet: Sequencing Flexible Configurations Figure 2: Advanced Patterned Flow Cell Design Enables UltraHigh Throughput. Patterned flow cells contain billions of nanowells at fixed locations, providing even cluster spacing and uniform feature size to deliver extremely high cluster density. Proven Technology, Exceptional Data Quality To deliver high data quality, the HiSeq 3000/HiSeq 4000 Systems leverage the proven SBS chemistry used in all Illumina sequencing systems, including the widely adopted HiSeq 2500 System. SBS chemistry relies on a proprietary reversible...

Specification Sheet: Sequencing Complete Sequencing Solutions lllumina offers a comprehensive portfolio that crosses the entire sequencing workflow, from library preparation to data analysis (Figure 3). TruSeq DNA and RNA Library Prep Kits support whole-genome sequencing, exome sequencing, and RNA-Seq on the HiSeq 3000/HiSeq HiSeq 3000/4000 Reagent Kits provide ready-to-use reagents for cluster generation and sequencing, reducing hands-on time and potential errors. Three different HiSeq 3000/4000 SBS Kits are available for use on the HiSeq 3000/HiSeq 4000 Systems. The SBS kits come multiple...

Specification Sheet: Sequencing Ordering Information. HiSeq 4000 Sequencing System SY-401 -4001 HiSeq 3000 Sequencing System SY-401 -3001 HiSeq 4000 System Upgrade SY-401 -4002 Reagent Kits HiSeq 3000/HiSeq 4000 System Specifications. Instrument Configuration Computer and touch screen display Installation setup and accessories Data collection and analysis software Instrument Control Computer Base Unit: 2x Intel Xeon E5-2697V2 64 BIT 2.7 GHz CPU Hard Drive: 8 x 1.0 TB SATA 7200 RPM 3.5" Constellation ES.3 (2 RAID 0 drives, 6 RAID 5 drives) Solid State Drive: 5 x 400 GB (5 RAID 0 drives)...