NextSeq 500 System
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High-throughput scale. Desktop simplicity. NextSeq™ 500 System. Flexible power. Speed and simplicity for whole-genome, exome, and transcriptome sequencing.

Harness the power of next-generation sequencing. In your lab. On your schedule. Introducing the NextSeq 500 Sequencing System—the first and only desktop sequencer to offer exome, transcriptome, and whole-genome sequencing. It’s the perfect combination of high-throughput sequencing, flexibility, and accuracy, all at a cost that makes it just right for your lab. Now it’s easier than ever to take advantage of the highest data quality by leveraging the world’s most widely used sequencing techno

Imagine the possibilities. Maximum flexibility. Maximum applications. Cancer discovery, profiling, and monitoring. • Uncover critical insights • Examine all stages of tumor progression • Envision the bigger picture quickly and efficiently • Look deeper and wider with whole-genome studies, targeted gene profiling, and gene expression Genetic disease variant discovery. • Explore the underpinnings of genetic disease • Find answers faster and more efficiently • Enable cytogenetic and copy number analysis Application flexibility. • Perform de novo sequencing to generate reference genomes •...

Scalable sequencing power. Better answers. Limitless discovery. The power of high-throughput sequencing. The simplicity of a desktop sequencer. With the NextSeq 500 System, there are no compromises. Exome Sequencing • Fastest sample-to-data exome solution • Most accurate variant detection • Comprehensive exome coverage RNA Sequencing • Hypothesis-free transcriptome analysis • Isoform characterization • Non-coding RNA analysis Whole-Genome Sequencing • High-quality, high-coverage genome • Unrestricted view of the genome now and in the future • Scalable, affordable whole human genome...

The power of on-demand sequencing. Total flexibility. Less waiting. Your needs can change from day to day. So can the NextSeq 500 System. For each run, you can choose between two flow cell sizes and accompanying reagents to shift between applications that require more or less sequencing output or smaller batch sizes. The NextSeq 500 System is scalable and flexible, supporting multiple applications with both low- and high-throughput capabilities. High-output kits   Up to 400 M reads Whole human genome Gene expression profil

Mid-output kits   Up to 130 M reads Enrichment panels Amplicon panels Industry-leading data quality. Proven technology. Highest confidence. Your studies deserve the best data quality. That’s what the NextSeq 500 System delivers. It’s no wonder that more than 90% of the world’s sequencing data are generated using sequencing by synthesis (SBS) technology from Illumina. Your study will benefit from: • The greatest accuracy, with > 80% of sequenced bases over Q30 • The highest percentage and yield of error-free reads • Fewer false positives, false negatives, and miscalls Gene expre

Streamlined bioinformatics. Easier analysis. Faster results. It’s never been easier to analyze your data. With the NextSeq 500 System, primary data analysis, including base calling and quality scoring, is performed using on-board computers. Then it’s easy to stream sequencing data into BaseSpace®, the Illumina genomics computing environment for data analysis, storage, and collaboration. With BaseSpace Cloud or BaseSpace Onsite you can perform: • Alignment • Variant detection • Annotation • Visualization • Interpretation The environment is secure. Access is easy. Analysis time is reduced....

Everything you need. Better technology. A community of support. The NextSeq 500 System surrounds you with everything you need for success. From initial setup through analysis, our service team of more than 300 professionals stands ready to serve you. You will be a part of the Illumina Community of more than 60,000 scientists using Illumina technology. Throughout the year, Illumina hosts user group meetings, symposiums, online forums, and more—all designed to bring researchers together to share ideas and advance science. The Illumina support and training teams are there to help you as your...