NextSeq 500
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System Specification Sheet: Sequencing NextSeq™ 500 Sequencing System A fast, flexible high-throughput desktop sequencer enabling a wide range of applications with the accuracy of Illumina SBS technology. Highlights Figure 1: NextSeq 500 Sequencing System • First High-Throughput Desktop Sequencer Performs whole-genome, exome, transcriptome sequencing and more, today or as your research grows • Highly Flexible to Fit Research Demands Supports a broad range of sequencing applications and offers tunable read length and multiple output configurations • Fast Turn-Around Time Rapid sequencing for time-critical studies ensures projects are completed in record time • Industry-Leading Illumina Chemistry Highly accurate SBS chemistry delivers high-quality results with no homopolymer issues • Push-Button Operation and Easy Data Analysis Walkaway sample-to-results solution with streamlined informatics performed on premises or in the cloud • End-to-End Illumina Scientific Support Illumina scientists and engineers are there every step of the way, providing installation, training, applications, and data analysis support Introduction A transformative addition to the industry-leading Illumina nextgeneration sequencing (NGS) system portfolio, the NextSeq 500 System delivers the power of high-throughput sequencing with the simplicity of a desktop sequencer (Figure 1). Its fast, integrated, sample-to-results workflow enables rapid sequencing of exomes, whole genomes, and transcriptomes in a single run, with the flexibility to switch to lower throughput sequencing as needed (Figure 2). The system fits seamlessly into research laboratories, with no need for specialized equipment. Illumina scientists are available at every point along the way with support and guidance, enabling researchers to focus on making the next breakthrough discovery. Enabling New Discoveries The NextSeq 500 System enables researchers to keep pace with technology, putting them in control of their sequencing projects. This robust, scalable system turns a broad range of high-throughput applications into affordable everyday research tools. The NextSeq 500 System’s flexibility enables researchers to switch quickly from one application to another and configure output based on sample volume and coverage needs. Now, even the smallest laboratory can perform any combination of sequencing applications to advance their studies, supporting faster publication of landmark research. By leveraging the latest advances in SBS chemistry and the industry’s simplest workflow, the NextSeq 500 System delivers high-quality results for whole-genome, exome, or transcriptome sequencing. Fast and Easy Workflow The NextSeq 500 System’s intuitive user interface and load-andgo operation enables researchers to perform more sequencing applications at the highest depth and resolution. It takes less than 10 minutes to load and setup the NextSeq 500 System. While other platforms require several pieces of specialized equipment, the NextSeq 500 System integrates cluster generation and sequencing into a single instrument and offers a seamless transition for onsite or cloud-based data analysis. Samples prepared using one of the simple, streamlined Illumina sample preparation kits are loaded into the NextSeq 500 System where sequencing is automated and fast. Data is generated in as little as 12 hours for a 75 cycle sequencing run and less than 30 hours for paired 150 cycle reads. Figure 2: NextSeq 500 System Sample-to-Answer Workflow* Prep 5.5 hours hands-on Sequence 10 minutes hands-on 5 minutes hands-on, on-site or in cloud Secure, unlimited storage The NextSeq 500 System’s simple workflow delivers highly accurate sequencing data. * Times vary by experiment and assay type. Details shown are for an mRNA expression profiling experiment assuming 2 × 75 bp on instrument, analysis results include differential expression and identification of alternative transcripts.

System Specification Sheet: Sequencing -Table 1: Unprecedented Flexibility for Multiple Applications By employing the lllumina industry-leading sequencing by synthesis (SBS) chemistry and file format conventions, the NextSeq 500 System offers customers access to the broadest ecosystem of established protocols, workflows, data sets, and data analysis tools. Industry-Leading SBS Chemistry Delivers Highest Accuracy At the core of the NextSeq 500 System is proven lllumina SBS chemistry, the most widely adopted NGS technology. This proprietary reversible terminator-based method enables the...

System Specification Sheet: Sequencing The NextSeq 500 System provides integrated support for paired-end sequencing, offering user-defined read lengths up to 2 × 150 bp. Join the Illumina Community The system is supported by the full suite of Illumina sample preparation and target enrichment solutions, offering library compatibility across the Illumina sequencing portfolio. This allows researchers to scale-up studies easily to the higher throughput HiSeq® System or perform follow-up studies on the MiSeq® System (Figure 3). With a NextSeq 500 System in their laboratory, researchers join a...

System Specification Sheet: Sequencing ■ NextSeq 500 System Specifications Instrument Configuration RFID tracking for consumables Instrument Control Computer (Internal)** Base Unit: Dual Intel Xeon ES-2448L 1.8 GHz CPU Operating System: Windows 7 embedded standard "Computer specifications are subject to change. Operating Environment Humidity: Non-condensing 20%-80% relative humidity Altitude: Less than 2,000 m (6,500 ft) Air Quality: Pollution degree rating of I Light Emitting Diode (LED) 520 nm, 650 nm; Laser diode: 780 nm, Class lllb Power Requirements Radio Frequency Identifier (RFID)...