Specification Sheet: Array Instruments HiScan® and iScan Systems Cutting-edge array scanners supporting rapid, sensitive, and accurate imaging of Illumina BeadChips for industry-leading genetic analysis results. Highlights • Highest Data Quality Proven Infinium®, GoldenGate®, and gene expression assays produce high call rates and provide industry-leading coverage • High Sample Throughput Fast optical scanners dramatically reduce scan times; optimized automation options maximize output • Flexible Multiple formats, applications, and automation options Flexible, Scalable Systems As array products from Illumina increase in complexity, with higher densities and a greater number of features, it is important to have a scanner that keeps pace. The cutting-edge HiScan and iScan systems are array scanners that support rapid, sensitive, and accurate imaging of BeadArray™ technology–based genetic analysis products (Table 1). Illumina scientists and engineers have developed these high-resolution scanners to deliver industry-leading data quality for a broad range of applications with the flexibility to meet a variety of throughput needs. The HiScan and iScan systems support the broad portfolio of innovative Illumina assays for genotyping, CNV analysis, DNA methylation, and gene expression profiling. Automating assay processing with a liquid-handling robot and the AutoLoader 2.x reduces hands-on time and enables scanning to occur 24 hours per day. Scanners and components are modular, creating a tunable system that can be configured to meet any level of throughput needed. Innovative Imaging System Both the HiScan and iScan systems use high-performance lasers, optics, and detection systems to offer submicron resolution and unmatched throughput rates. The result is industry-leading scan times without sacrificing data quality and reproducibility. The latest HumanOmni BeadChips have been optimized to take advantage of the higher performance components of the HiScan system, offering industry-leading throughput, and the highest number of high-value content markers. With a high signal-to-noise ratio, high sensitivity, low limit of detection, and broad dynamic range, both scanners produce exceptional data quality for use in any biomarker screening or validation study. The high call rates (> 99% achieved with the Infinium Assay) enable powerful genome-wide association studies and high-resolution CNV analysis, accurately detecting even single copy number changes. In addition, these systems can be used for fast, accurate screening in agrigenomics and for validating complex disease studies. Methylation and gene expression profiling also benefit from the sensitive measurement and wide dynamic range. Table 1: HiScan and iScan Systems at a Glance System Avg. scan time per sample (HumanOmni2.5-8 BeadChip) Avg. samples per week* (manual)† (HumanOmni2.5-8 BeadChip) Avg. samples per week (automated)‡ (HumanOmni2.5-8 BeadChip) * Five-day work week. † Manual: 1 FTE, 1 HiScan or iScan system, 16 BeadChips/batch, 2 batches/FTE/Tecan robot. ‡ Automated: 1FTE, 1 HiScan or iScan system, 2 Tecan robots, 1 AutoLoader 2.x, 24 BeadChips/batch, 2 batches/FTE/Tecan robot.

Specification Sheet: Array Instruments Table 2: Weekly Throughput of Selected Illumina BeadChips with a Single HiScan or iScan System Approximate Scan Time Per Sample (minutes) Manual Loading* (Samples Per Week) With Automation† (Samples Per Week) HiScan Infinium HumanOmniExpress+ Universal-32 BeadChip for GoldenGate Genotyping * Manual: 1FTE, 1 HiScan or iScan system, 16 BeadChips/batch, 2 batches/FTE/Tecan robot per week. Automated: 1FTE, 1 HiScan or iScan system, 2 Tecan robots, 1 AutoLoader 2.x, 24 BeadChips/batch, 2 batches/FTE/Tecan robot per week. High-Throughput Readout Fully Automation...

Specification Sheet: Array Instruments LIMS Integration Accurate sample information, workflow enforcement, and data tracking are ensured with an optional integrated Infinium or GoldenGate Laboratory Information Management System (LIMS) designed specifically for lllumina products. Illumina LIMS features an easy-to-use custom interface, positive sample tracking (posID), and tools to manage entire projects. Samples are validated and followed throughout the workflow to ensure correct assay processing. Integrated tools support project manage- ment tasks such as managing concurrent projects, tracking...

Specification Sheet: Array Instruments The cutting-edge HiScan and iScan array scanners support a wide breadth of applications. For the highest throughout and fastest time to answer, choose the HiScan system. The iScan system offers an accessible entry point into high-throughput array studies, with no compromises in terms of data quality or breadth of applications. Both systems include industry-leading support, ensuring that researchers get the most out of their array system for superior genetic analysis studies. To learn more about lllumina genetic analysis products, visit Ordering Information"...