Specification Sheet: Sequencing MiniSeq™ System The power of proven Illumina sequencing, now more accessible than ever. Highlights • Accessible Illumina Sequencing Affordable-to-acquire and cost-efficient to run, even with low numbers of samples • Push-Button Operation and Easy Data Analysis Walk away library-to-results solution with onboard data analysis • Highly Flexible to Fit Research Demands Supports a broad range of DNA and RNA sequencing applications for examining single genes to entire pathways Introduction The MiniSeq System (Figure 1) delivers the quality and reliability of Illumina next-generation sequencing (NGS) technology in our most affordable benchtop sequencer to date. It enables researchers to keep pace with technology and take control of their sequencing projects. This small, robust system turns a broad range of NGS methods into affordable, everyday research tools. With cost-efficient operation, even for low numbers of samples, there is no need to wait to batch samples for sequencing on a high-throughput instrument. With the MiniSeq System, researchers can sequence on demand. It circumvents the iterative, time-consuming testing of Sanger sequencing and qPCR to allow for interrogation of single genes to entire pathways with full-gene coverage. Now, laboratories of any size can perform a range of sequencing methods to deliver results and advance their studies. Powerful Sequencing Made Simple The MiniSeq System features a simple, integrated, library-toresults workflow that enables sequencing of both DNA and RNA with minimal hands-on time (Figure 2). It is ideal for targeted research applications like cancer sequencing and gene expression profiling. Onboard, touch-screen data analysis with a simple, intuitive user interface eliminates the need for specialized equipment or bioinformatics expertise. The MiniSeq System has the smallest footprint of any Illumina sequencing system, allowing it to fit seamlessly onto laboratory benchtops (Figure 3). Illumina scientists are available at every point along the way with support and guidance, enabling researchers to focus on making the next breakthrough discovery. For Research Use Only. Not for use in diagnostic procedures. Figure 1: MiniSeq System—By harnessing the latest advances in SBS chemistry and simple, streamlined workflows, the MiniSeq System delivers a library-to-results solution that is more affordable than ever. Streamlined Sequencing Workflow The MiniSeq System provides an intuitive user interface and load-and-go operation, making it easy to learn and easy to use. It integrates clonal amplification, sequencing, and data analysis into a single instrument, eliminating the need to purchase and operate specialized, ancillary equipment. After library preparation using a simple, streamlined Illumina library prep kit, libraries are loaded into the MiniSeq System where sequencing is automated. It takes less than 5 minutes to load and set up a run on the MiniSeq System. Runs are complete in < 1 day, and data analysis is performed onboard the instrument or in the BaseSpace® platform - the Illumina genomic computing environment. A suite of data analysis tools, and a growing list of third-party BaseSpace Applications (Apps), empowers researchers to perform their own informatics analysis easily. By employing the industry-leading Illumina sequencing by synthesis (SBS) chemistry and file format conventions, the MiniSeq System offers customers access to a broad ecosystem of established protocols, workflows, data sets, and data analysis tools. Prepare Library Analyze Data MiniSeq Sequencing • End-to-End Support Illumina scientists and engineers provide installation, training, and support, from assay design through data analysis 5 minutes hands-on, on-site or in cloud Instantly Secure, limited storage Figure 2: MiniSeq System Sequencing Workflow—The MiniSeq System offers a simple, integrated workflow from library preparation to onboard data analysis. Workflow times will vary by experiment and assay type. Details shown are for a TruSeq® Custom Amplicon sequencing experiment assuming 2 x 150 bp o

Specification Sheet: Sequencing Table 1: Flexibility for Multiple Applications Application Targeted Amplicon Sequencing 750 amplicons 1000× coverage 2 × 150 bp Targeted Expression Profiling 65 targets 1 × 50 bp Enrichment Panel 1 Mb Region 100× coverage 2 × 75 bp microRNA Sequencing 5 M reads/sample 1 × 36 bp Small Whole Genome Sequencing 5 Mb Genome 30× coverage 2 × 150 bp Industry-Leading SBS Chemistry Delivers High Accuracy At the core of the MiniSeq System is industry-leading Illumina SBS chemistry, the most widely adopted NGS technology worldwide.* This proprietary reversible terminator–based...

Specification Sheet: Sequencing Table 2: MiniSeq System Performance Parameters MiniSeq System Sequencing Performance8 Flow Cell Configuration Read Length (cycles) Required Input Library Prep Kits a. Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter b. Times include cluster generation, sequencing, and base calling with quality scores on a MiniSeq System c. The percentage of bases > Q30 is averaged over the entire run. BaseSpace Environment (Cloud or Onsite). Downstream data analysis using the BaseSpace platform includes alignment and variant...

Specification Sheet: Sequencing Ordering Information System Name MiniSeq System MiniSeq High Output Kit (75 Cycles) MiniSeq High Output Kit (150 Cycles) MiniSeq High Output Kit (300 Cycles) MiniSeq Mid Output Kit (300 Cycles) Learn More To learn more about the next revolution in sequencing, visit Maximize Performance and Productivity with lllumina Services, Training, and Whether immediate help is needed during an instrument run, or in-depth consultations are required for sophisticated workflows, lllumina can help, lllumina service and support teams provide a full suite of expedient, customized...