Simplest, fastest. For every lab.Meet the next generation of benchtop sequencing systems MiSeq™ ilOO and MiSeq ilOO Plus Sequencing Systems M-GL-02245 v3.0 For Research Use Only. Not for use in diagnostic procedures. illumina

Setting the standard for sequencing simplicity and speed For Research Use Only. Not for use in diagnostic procedures.

Simplest, fastest. For every lab. With the MiSeq i100 Series, Illumina continues to set the highest standards. Advancements in system design, sequencing chemistry, and data analysis deliver the simplest, fastest benchtop sequencing and proven accuracy. The MiSeq i100 Series is accessible to users of all levels and simplifies the NGS workflow—from library preparation to data analysis. With same-day results for various applications, including small whole-genome sequencing (WGS), targeted gene sequencing, and gene expression analysis, the MiSeq i100 Series enables labs to address complex genomics...

Unthinkable speed for same-day results The MiSeq i100 and MiSeq i100 Plus Systems are designed to decrease turnaround time four-fold compared to the MiSeq System. With sequencing run times as fast as four hours, you can achieve same-day results to discover more, faster. The MiSeq i100 Series are powered by XLEAP-SBS chemistry, the fastest, most accurate, Illumina sequencing by synthesis (SBS) chemistry to date. Improved robustness and stability of XLEAP-SBS reagents allow for shipping and storage at room temperature, expediting run setup without waiting for reagents to thaw. TM The MiSeq i100...

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

Remarkably simple sequencing from setup to data analysis Sequencing is easier than ever with streamlined operations that reduce the required time and resources and improve efficiency throughout the entire run. Simplified run setup takes three steps and less than 20 minutes. Enhanced usability features, including load-and-go consumables, onboard denaturation, onboard cluster generation, and no post-run washing, streamline the sequencing workflow. Onboard DRAGEN secondary analysis provides highly accurate and efficient data analysis, reducing reliance on bioinformatics experts to overcome bottlenecks...

Diverse applications with flexible output With the MiSeq i100 Series, you can access 10 configurations across multiple flow cell options with read lengths up to 2 × 300 bp to support an output range of 5–100 million reads and 1.5–30 Gb. Readily increase sample throughput and perform deeper sequencing to support your applications from targeted gene sequencing to small WGS and more. For Research Use Only. Not for use in diagnostic procedures. Flexible output Ten reagent configurations with read lengths up to 2 x 300 bp for a wider output range Increased output Data output of 1.5–30 Gb and 5–100...

For Research Use Only. Not for use in diagnostic procedures.

Groundbreaking science meets groundbreaking sustainability The MiSeq i100 Series is purposefully designed to reduce environmental impact. Combined with innovative XLEAP-SBS reagents, these instruments deliver remarkable sustainability and user experience benefits: Room-temperature storage No thawing required, expediting setup time and eliminating need for freezers to reduce carbon footprint Minimizes time spent unboxing, simplifying disposal and reducing the environmental impact Room-temperature shipping 35% carbon emission reduction† Requires no dry ice and no ice packs for less waste Minimizes...

For Research Use Only. Not for use in diagnostic procedures.

Get trusted results, faster The MiSeq i100 Series offers sample-to-analysis workflow solutions. These workflows include library preparation, sequencing on the MiSeq i100 Series, and onboard analysis. Preconfigured DRAGEN secondary analysis provides comprehensive data summaries for select infectious disease, microbiology, and oncology applications in under two hours. Streamline experimental planning and setup by choosing from preselected library prep kits and targeted gene panels. Simplify data analysis with access to preconfigured DRAGEN pipelines either onboard or in the cloud, minimizing the...

Microbial genomics Pathogen detection 16S metagenomics Shotgun metagenomics Small WGS Transcriptomics 3′ gene expression Targeted RNA panel mRNA-Seq Total RNA-Seq Gain insights into how microbes impact humans and the environment Targeted gene sequencing Amplicon-based NGS Enrichment-based NGS Gene editing Immune repertoire transcripts of interest to perform gene expression and fusion detection studies Quality control Library QC Assess library quality before committing to a full-scale run Focus on a select set of genes of interest for deep sequencing NGS workflows The MiSeq i100 Series offers...

INSTRUMENT SERIES Two instrument configurations The MiSeq i100 and MiSeq i100 Plus Sequencing Systems set new standards for simplicity and speed, with exceptional accuracy. They’re designed with a flexible, easy-to-use sequencing workflow. MiSeq i100 Plus Sequencing System Outputs ranging from 1.5 to 30 Gb Read lengths from 1 × 100 bp to 2 × 300 bp MiSeq i100 Sequencing System Outputs ranging from 1.5 to 15 Gb Read lengths from 1 × 100 bp to 2 × 300 bp For Research Use Only. Not for use in diagnostic procedures. MiSeq i100 Plus System

Single reads Paired-end reads Flow cell type Output Reads passing filter per flow cell For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

Support that never stops Illumina strives to be the best partner possible. With a global presence, you can rely on our experts to facilitate your success. Technical support is available via phone 5 days a week or access online support 24 hours, 7 days a week, worldwide and in multiple languages, with rapid response times near most major metropolitan areas. Illumina provides excellent product consistency, supply, and quality enabled by a mature global manufacturing infrastructure. The MiSeq i100 Series can be connected to the free Illumina Proactive, a secure and remote instrument performance...