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virotype BVDV 2.0 workflow

virotype BVDV 2.0 workflow

virotype BVDV 2.0 workflow

Product catalog summary
Introduction
INDICAL BIOSCIENCE has developed the virotype BVDV 2.0 RT-PCR Kit, a molecular solution for the control of Bovine Viral Diarrhea Virus (BVDV), used by veterinary diagnosticians worldwide. This kit builds on 15 years of experience and offers enhanced protocols and ease of use.

Key Features
  • Faster Results: The RT-qPCR runtime is approximately 1 hour.
  • Sensitivity and Specificity: The kit uses a next-generation triplex real-time RT-PCR assay for reliable identification of all known BVDV genotypes.
  • Internal Controls: Includes both endogenous (β-actin) and exogenous internal controls for greater confidence.
  • Ease of Use: Ready-to-use reagents with a single reaction mix save lab time.
  • Sample Types: Validated for blood, plasma, serum, tissue, milk, and ear tissue from cattle.
  • Pooling Options: High sensitivity allows testing of blood and milk samples in pools of up to 100, and ear tissue samples in pools of up to 25.

Workflow and Equipment
  • IndiMag 2: A benchtop instrument for automated nucleic acid extraction, capable of processing up to 48 samples simultaneously.
  • IndiMag Pathogen Cartridges: Prefilled reagent cartridges for viral RNA/DNA and bacterial DNA isolation using magnetic bead technology.
  • virotype Tissue Lysis Reagent: Facilitates fast lysis of bovine ear tissue samples.

Performance Data
The document includes a table showing Ct values for individual and pooled samples, demonstrating the kit's effectiveness in detecting BVDV RNA in various sample types.

Product Information
  • virotype BVDV 2.0 RT-PCR Kit: Available in 100 and 500 reaction formats.
  • IndiMag Pathogen Kits: Various configurations for different sample processing needs.

Regulatory and Contact Information
The products are for veterinary use only and are subject to regulatory requirements that vary by country. For more information, visit INDICAL's website or contact their support team.
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Catalog excerpts

virotype BVDV 2.0 workflow-1

virotype BVDV 2.0 workflow Veterinary diagnosticians worldwide rely on INDICAL‘s molecular solutions for Bovine Viral Diarrhea Virus (BVDV) control, using it to test millions of calves annually. Building upon 15 years of experience, we have developed the virotype BVDV 2.0 RT-PCR Kit (FLI-C 112) with increased ease of use and enhanced protocols. virotype BVDV 2.0 RT-PCR Kit he benchtop powerhouse for automated T nucleic acid extraction IndiMag prefilled cartridges or flexible, automated, magnetic F bead-based extraction and purification of BVDV-RNA using 96-well plate platforms or the IndiMag 48s/IndiMag 2 aster time to result F RT-qPCR runtime of ~1 hour* old standard sensitivity and specificity G Next-generation triplex real-time RT-PCR assay for reliable identification of all known BVDV genotypes dditional internal controls for greater confidence A Endogenous Internal Control (β-actin) and Exogenous Internal Control I ncreased ease of use Ready-to-use reagents including a single reaction mix, saving precious lab time ultiple validated sample types M Blood, plasma, serum, tissue, milk, and ear tissue from cattle E xtended pooling options:** virotype Tissue Lysis Reagent ysis buffer for fast, convenient and L inexpensive preparation of RNA from ear tissue samples Blood, plasma, serum, milk * See virotype BVDV 2.0 RT-PCR Kit Handbook for detailed information. ** The high sensitivity of the virotype BVDV 2.0 RT-PCR Kit permits blood samples (whole blood, plasma, serum) and milk samples to be tested in pools consisting of up to 100 individual samples, and ear tissue samples in pools of up to 25 individual samples. Pool recommendations and regulations may vary

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virotype BVDV 2.0 workflow-2

BVDV RNA can be reliably detected with the virotype BVDV 2.0 RT-PCR Kit in pools of up to 100 serum and blood samples. number material sample 50 100 Sample Sample Individual Pools of Pools of number material sample 50 100 Table: Pools of 50 and 100 were generated by diluting BVDV-positive serum and EDTA blood samples in BVDV-negative bovine serum and extracted using the IndiMag Pathogen IM48 Cartridge. Product Short description reactions/preps) virotype BVDV 2.0 Real-time RT-PCR assay for identification of RNA RT-PCR Kit* from Bovine Viral Diarrhea Virus (BVDV). A perfect match: Get the best...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.