Walk-away DNA/RNA sample preparation

SPECIFICATIONS AND ORDERING INFORMATION

SUPERIOR AUTOMATED SAMPLE PREPARATION Magnetic separation module for binding of magnetic particles Simple operation via touchscreen for protocol selection and system set up Built-in PC with unidirectional LIMS connectivity Sample and reagent loading racks for barcoded labware with integrated barcode reader Waste station The InviGenius® PLUS is a walk-away system for nucleic acid purification for up to 12 samples from up to 4 ml of sample volume. Innovative functionality and optimized protocols for demanding samples and applications result in reliable performance and superior DNA and RNA quality...

INVIGENIUS® PLUS DECK LAYOUT Magnetic separation head 3 positions for disposable tip racks Eluate position for a microplate Incubator with microplate position Position for a disposable sheaths rack Working position for a microplate with the possibility to heat the foremost row Loading bay lanes for a sample rack, an eluate rack and a reagent rack THE NEW PLUS FEATURES 1. Deep-well working plate for sample volumes of up to 4 ml 2. Heat lysis and heat elution for high recovery rates of nucleic acids from demanding samples such as liquid biopsies or virus samples 3. Barcoded labware for complete...

The InviGenius® PLUS delivers exceptional process safety in a completely monitored operation environment. Elaborate software control and intuitive user guidance enable full process control and documentation and prevent sample tracking errors. 1. LOADING RACKS FOR PRIMARY TUBES Integrated barcode reader for sample identification during loading • Prevention of human errors • Tracking of samples • Connectivity with LIMS allowing workflow continuity and archiving of the data Examples of compatible primary tubes: • Sarstedt Monovette® • BD Vacutainer® • TERUMO Venosafe® • Greiner Bio-One Vacuette®...

3. PLUG-IN FOR SEPARATE HAND-HELD BARCODE SCANNER • Choice of elution tubes or plates • Complete traceability for all plastic ware 4. SOFTWARE-SUPPORTED SETUP OF REAGENTS,WASTE AND DISPOSABLES • Inventory checks before and during run • Defined and protected protocols prevent user-errors 5. AIR DISPLACEMENT PIPETTOR • Barometric and conductive liquid level detection • Reduced maintenance and waste - no system liquid is required • Prevention of cross contamination through use of filter tips and intelligent routing Aliquot pipetting of eluates for generation of replicates, e.g. for PCR Pre-mix of...

FULLY AUTOMATED, WALK-AWAY DNA/ RNA PURIFICATION The InviGenius® PLUS is a true walk-away system for DNA/RNA extraction and purification from clinical samples - providing a reliable “Sample in - Eluate out” technology! 1. HEAT INCUBATOR • Automated heat lysis (up to 95 °C) on board • A heated lid prevents aerosol formation and condensation • Suitable for deep-well working plate for sample volumes of up to 4 ml 2. MAGNETIC SEPARATION MODULE • Twelve magnetic rods transfer magnetic beads and mix samples ■ Disposable sheath racks for several runs • Bottom magnets prevent carry-over of beads into...

3. WASTE MANAGEMENT AND DECONTAMINATION • Used tips and sheaths will be disposed into the waste container; Pipettor drips excessive liquid into it • Waste container is rotating slowly for better utilization and can be removed and disposed • UV light enables decontamination of the worktable Advanced troubleshooting • Acoustic signal, e.g. in case of closed bottles, clotted sample • Flagging of problematic samples, e.g. clots • Automatic hard disk space management

NUCLEIC ACID EXTRACTION PRINCIPLE The InviGenius® PLUS controls an array of magnetic rods that can collect or release magnetic particles. After sample lysis the nucleic acids are bound to the magnetic particles and transferred through the extraction, purification, and elution processes. This circumvents pipetting errors. The eluted pure nucleic acids are ready-to-use for subsequent downstream applications. collection of magnetic beads with bound DNA release of magnetic beads with bound DNA collection of magnetic beads with bound DNA „switched off“ magnetic rods „switched on“ magnetic rods „switched...

Nucleid Acid Starting Material Product Name Genomic, bacterial, viral DNA & viral RNA 200 µl whole human blood (EDTA, citrate), plasma, serum, cell-free body fluids, rinse liquid from swabs, supernatant from stool suspensions, sputum, BAL, urine 200 µl whole human blood (EDTA, citrate) 2 ml whole human blood (EDTA, citrate) InviMag® Blood DNA Mini Kit/ IG InviMag® Blood DNA Maxi Kit/ IG 1.6 ml SalivaGene® stabilized saliva samples FFPE tissue, fresh biopsy samples, cryosections up to 4 ml plasma, serum, urine InviMag® Free Circulating DNA Kit/ IG InviMag® Bisulfite Conversion Kit/ IG The InviMag®...

1. AUTOMATION FOR ONCOLOGY a) EXTRACTION OF CELL-FREE CIRCULATING DNA The InviMag® Free Circulating DNA Kit/ IG enables efficient, fully automated purification of cell-free circulating DNA (cfDNA) fragments from 4 ml of plasma or serum samples on the InviGenius® PLUS. Fig. 1: Excellent and reproducible intra-run recovery of circulating cfDNA Circulating cell-free DNA was isolated from 12 aliquotes of 4 ml Seracon plasma samples in parallel using the InviMag® Free Circulating DNA Kit/ IG on the InviGenius® PLUS and eluted in 100 µl. DNA yield was quantified by real-time PCR of two amplicons within...

Total Allele Allele Total Allele Allele Sample Gene Read Read Read Molecular Molecular Molecular Name ID Allele Name Variant Coverage Coverage Frequency Coverage Coverage Frequency Plasma samples from different cancer patients (Breast Cancer, Non-Small Cell Lung Cancer, Small Cell Lung Cancer, Metastatic Melanoma) were used as starting material. cfDNA was isolated from 4 ml of plasma into an elution volume of 80 yl using the InviMag® Free Circulating DNA Kit/ IG on the InviGenius® PLUS in comparison to a manual kit Q. Targeted Next Generation Sequencing (Oncomine cfDNA assay, Thermofisher) showed...