CW3125H-3 Inactivated VTM
2Pages

Viral Sample Preservation Solution (Inactivated) It contains inactivated virus components to ensure the safety of transportation and testing personnel. Nucleic acid can be stored at room temperature for up to 7 days. There is no significant effect on the integrity of viral nucleic acid with high temperature inactivation treatment. Product Characteristics ● Ensuring Safety: It can inactivate the virus, eliminate secondary infection, and ensure transportation and detection safety. ● Transportion at normal temperature:Viral RNA can be kept for one week at 37°C without degradation. It is suitable for long-distance transportation, ensuring the detection accuracy and less transportation cost.. ● Protect viral nucleic acid from high temperature inactivation: Treatment at 60°C for 30min before extraction has no significant impact on the integrity of viral nucleic acid. Reliable nucleic acid preservation ability The culture of IBV was added to the CoWin’s preservation solution , and the control group was set as follows: After stored at -20°C for 7 days, the culture was diluted to 10-1, 10-2, 10-3, 10-4, 10-5. Experimental group: Stored at 37°C for 7 days, the culture was diluted to 10-1, 10-2, 10-3, 10-4, 10-5. 200 ul samples were collected for extraction RNA and Black：-20℃ for 7d The culture of IBV was added to the CoWin and virus sample preservation solution of two guanidine, placed at -20°C and 37°C for 0-7 days CoWin Preservation solution containing guanidine hydrochloride respectively, and 200 ul samples were collected for extraction RNA Preservation solution containing guanidine thiocyanate and was using RT-qPCR to detection. The results showed: The CoWin’ virus sample preservation solution could effectively preserve the nucleic acid without degradation within seven days, and the preservation efficiency was better than others virus sample preservation solution. www.cwbiosciences.com

Inactivating virus to ensure safety Chicken infectious bronchitis virus QXL87 was added to the preservation solution, and the virus solution was inoculated into 10 day old SPF chicken embryonated eggs by allantoic cavity inoculation at room temperature of 18-26°C for 45 min. The harvested allantoic fluid was tested for nucleic acid, according to GB / T 23197-2008, diagnostic technology for avian infectious bronchitis, RNA was extracted by CoWin’s RNA extraction kit, and nucleic acid was detected by RT qPCR. The number of dead chicken embryos and diseased live embryos were observed and...