CW3126 Column method Viral DNA/RNA Extraction Kit IFU

Product catalog summary

Manufacturer Information:
Jiangsu CoWin Biotech Co., Ltd, located in Taizhou, Jiangsu, China, is the manufacturer of the Viral DNA/RNA Kit. Their contact details include phone, fax, and email. The EC-Representative is SUNGO Europe B.V. based in Amsterdam, Netherlands.

Product Overview:
The Viral DNA/RNA Kit is designed for the isolation, enrichment, and purification of nucleic acids from various samples such as swabs, serum, plasma, and bronchoalveolar lavage fluid. The kit is available in sizes for 50 or 200 tests per box and is suitable for clinical in vitro diagnostics.

Applications:
This kit provides a rapid and efficient method for extracting DNA/RNA, which can be used in PCR and fluorescence quantitative PCR experiments. The unique buffer system ensures high purity and stable quality of nucleic acids, free from contaminants.

Storage and Validity:
The kit should be stored at 0-35°C for up to 12 months. During transportation, it should be kept at 0-40°C for no more than 7 days.

Sample Requirements:
Applicable samples include swabs, serum, plasma, and other cell-free body fluids.

Kit Components:
The kit includes lysis buffer, washing buffers, RNase-Free Water, adsorption columns, and collection tubes. Users need to supply a constant temperature mixer, isopropanol, and 100% ethanol.

Preparation:
Before starting, users must add isopropanol and ethanol to the respective washing buffers and mix all reagents gently.

General Purification Procedure:
1. Mix sample with lysis buffer and isopropanol, vortex, and shake.
2. Transfer to adsorption column, centrifuge, and discard liquid.
3. Wash with buffers, centrifuge, and dry.
4. Elute nucleic acids with RNase-Free Water and store at -80°C.

Rapid Purification Procedure (Swab Samples Only):
1. Mix swab sample with lysis buffer and isopropanol, vortex, and centrifuge.
2. Transfer to adsorption column, centrifuge, and discard liquid.
3. Wash with buffer, centrifuge, and elute nucleic acids with RNase-Free Water.

Catalog excerpts

CW3126 Column method Viral DNA/RNA Extraction Kit IFU-1

Phone : 86-523-86201352 Fax : 86-523-86816890 Email: [email protected] Ver.A/3 Notes 1. Please read this manual carefully before doing experiment. 2. Please check whether there is crystallization or precipitation in the lysis buffer before use. If it happens, please dissolve it in a 56‘C water bath. Viral DNA/RNA Kit Product: Viral DNA/RNA Kit Size: 50 tests/Box, 200 tests/Box Manufacturer Jiangsu CoWin Biotech Co., Ltd Add: No.58 G52 Building, East of Lujia Road, West Side of Tai Road, China medical city, Taizhou, Jiangsu, 225300 Phone: 86-523-86201352 Fax: 86-523-86816890 Web: https://www.cwbio.com E-mail: [email protected] EC-Representative SUNGO Europe B.V. VAT: NL857821659B01 Add: Olympisch Stadion 24, 1076DE Amsterdam, Netherlands Contact: SUNGO Secretary Tel /Fax: +31 (0) 2021 11106 E-mail: [email protected] Version: A/3 Effective Date: 2020-08-04 Applications It is used for nucleic acids isolation, enrichment and purification. The processed products can be used for clinical diagnosis in vitro. Principle Nucleic acid isolation and purification kit provides a simple, rapid and efficient method to extract DNA/RNA from swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids. The unique buffer system enables the nucleic acid in the lysate to be efficiently and specifically binded to the silica-based membrane. The obtained nucleic acid has high purity, stable quality, and is free of protein, nuclease and other contaminants and inhibitors. It can be applied to various conventional operations, including PCR, fluorescence quantitative PCR and other experiments. www.cwbio.com CoWin Biotech CoWin Biotech

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CW3126 Column method Viral DNA/RNA Extraction Kit IFU-2

Kit Components Storage Condition and Valid Period Store at 0-35°C up to 12 months. We suggest products transportation at 0-40°C for no more than 7 days. Sample Requirements Applicable Samples: swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids. Procedure Equipment and Reagents to Be Supplied by User: Constant temperature mixer (CWBIO,CW2593 is recommended), Isopropanol, 100% ethanol. Things to do before starting: a. Add isopropanol to Washing Buffer 1 (concentrate) and 100% ethanol to Washing Buffer 2 (concentrate) according to the label of the reagent bottle ,...

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