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CW3130M One-step COVID-19 qPCR kit Brochure

CW3130M One-step COVID-19 qPCR kit Brochure

Product catalog summary
Product Overview
The document describes a COVID-19 nucleic acid detection kit designed for rapid, sensitive, and easy-to-use testing.

Product Characteristics
  • Immediate Detection: The kit allows quick operation with a fluorescence quantitative PCR instrument, suitable for large-scale epidemic screening in communities, schools, airports, and other public areas.
  • High Sensitivity: The minimum detection limit is as low as 100 copies/ml, enabling detection of low viral loads.
  • Fast Processing: The entire process from sample collection to result takes approximately 40 minutes.
  • Room Temperature Transportation and Storage: The kit can be stored and transported at room temperature, reducing cooling costs. After opening, storage at 2-8°C is recommended.

Package and Contents
  • Each box contains 48 tests.
  • The kit includes all necessary components for direct amplification without RNA extraction.

Detection Method and Targets
  • Detection is performed using PCR-Fluorescence Probing technology.
  • Target genes detected are ORF1ab and N genes of SARS-CoV-2.

Sample Requirements
  • Samples can be collected via nasal or pharyngeal swabs.

Instrument Compatibility
  • Compatible with common qPCR instruments such as ABI 7500, SLAN-96S, LightCycler, and Bio-Rad CFX96.

Performance Metrics
  • Sensitivity: Minimum detection limit of 100 copies/ml.
  • Precision: Coefficient of variation (CV) within batch is ≤5% based on Ct values.
  • Specificity: No cross-reactivity observed with other coronaviruses (NL63, HKU1, 229E, OC43), influenza A and B viruses, or human genomic DNA. Negative controls were all negative.

Storage and Validity
  • Kit valid for 12 months.
  • Can be stored at room temperature before opening; recommended 2-8°C after opening.

Testing Procedure
  1. Collect sample using nasal or pharyngeal swab.
  2. Place swab head into virus sampling tube, shake and mix thoroughly.
  3. Take mixed liquid and add to amplification reaction tube.
  4. Insert tube into qPCR instrument and run detection.

Regulatory Status
The kit has FDA Emergency Use Authorization.

Summary
This COVID-19 nucleic acid detection kit offers rapid, sensitive, and specific detection with minimal sample preparation and no need for cold chain logistics. It is suitable for large-scale screening in various settings, providing reliable results within 40 minutes.
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Catalog excerpts

CW3130M One-step COVID-19 qPCR kit Brochure-1

Novel Coronavirus (SARS-CoV-2) Fast Nucleic Acid Detection Kit (PCR-Fluorescence Probing) Whole process only in 4 minutes Non-cold chain to minimize the cooling cost Contains all components from collection to detection Product Characteristics ● Immediately detectable: It’s easy to operate, without extraction, and only needs a fluorescence quantitative PCR instrument, The number of samples in a single test can be adjusted flexibly according to the number of people to be tested, which is extremely suitable for epidemic screening in communities, stations, airports, customs, supermarkets, schools and other areas. ● Hypersensitivity: The minimum detection limit can be as low as 00 copies / ml. ● Fast: Complete the whole process from sampling to detection only in 4 minutes. ● Transportation at room temperature : The kit can be stored at room temperature and non-cold chain to minimize the cooling cost. Detection Process 1. Collect the sample with a nasal or pharyngeal swab 2. Put the head of swab into the virus sampling tube, shake it up and down and mix well 3. Take the mixed liquid and add it to the reaction amplification tube 4. Put tube into the qPCR instrument for detection and determination of the result Product Performance Index Package Detection Methods PCR-Fluorescence Probing (direct amplification) Detect genes Sample Requirements nasal swab, pharyngeal swab Instrument Compatibility There is no cross-reactivity with positive samples such as coronavirus (NL63, HKU1, 229E, OC43), influenza A virus, influenza B virus Yamagata type, and human genomic DNA. The negative control of the testing enterprises were all negative. Minimum detection limit: 00 copies / ml The coefficient of variation (CV,%) of the precision Ct value within the batch was ≤5%. Storage Condition stored at room temperature,after the kit is opened and used, it is recommended to store at 2-8°C. Valid Period

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.