cfDNA Storage Tube It can be kept at 6~37°C for 14 days cfDNA protection solution- upgraded formula The upgraded protective liquid formula greatly improves the protection ability of cell-free DNA, and guarantees more accurate test results 1、Prevent hemolysis Protect red blood cells from rupture and reduce hemolysis. 2、Prevent genomic DNA contamination Protect leukocytes from rupture, and prevent genome DNA in leukocytes from releasing into plasma, causing cfDNA contamination. 3、Prevent cfDNA degradation It can inhibit the degradation of cfDNA by nuclease and keep the cfDNA in the blood for 14 days under the condition of 6-37 ° C, providing high-quality test samples for subsequent experiments Experimental Comparison Cowin S’company EDTA Figure 1. Blood samples were collected into CoWin’ preservation tube, S’ company preservation tube and EDTA anticoagulation tube respectively. After 0, 7 and 14 days at room temperature, the absorbance of cell-free hemoglobin was measured at 414nm. It can effectively fix blood cells and prevent the release of gDNA into plasma Concentration of the cfDNA in plasma (ng/ml) absorbance of free hemoglobin It can inhibit hemolysis during the storage and transportation Cowin S’company EDTA Figure 2. Blood samples were collected into CoWin’ preservation tube, S’ company preservation tube and EDTA anticoagulation tube respectively. After 0, 7 and 14 days at room temperature, cell-free DNA was extracted with CoWin’ CW2603 “CWhipro Circulating Nucleic Acid Kit”. The results showed that CoWin preservation tube could effectively inhibit hemolysis. The results showed that the blood cells preserved in tubes containing EDTA had been ruptured seriously and released a lot of DNA into plasma. CoWin’s preservation tube can effectively fix blood cells and prevent the release of genomic DNA into plasma. It Effectively inhibits nuclease activity and protects free DNA Establishing high quality DNA library Cowin S’company EDTA Figure 3. Blood samples were collected into CoWin’s preservation tube, S’ company preservation tube and EDTA anticoagulant tube respectively, and non-human internal reference gene of 150bp size was added. After 0, 7 and 14 days of room temperature treatment, qPCR was used to detect the expression level of internal reference gene. The results showed that the expression of internal reference genes in EDTA anticoagulant tube decreased over time. CoWin’s tube could effectively inhibit the activity of nuclease in plasma and protect cell-free DNA from degradation. Figure 4. Blood samples were collected into CoWin’s preservation tube, cell-free DNA was extracted from plasma and cfDNA library was established. DNA fragment distribution in the library was detected by highly sensitive nucleic acid analyzer. The results showed that high quality DNA library could be established by using the free DNA of CoWin’s tube.