Phone : 86-523-86201352 Fax : 86-523-86816890 Email: [email protected] Ver.A/2 Manufacturer Jiangsu CoWin Biotech Co., Ltd Add: No.58 G52 Building, East of Lujia Road, West Side of Tai Road, China medical city, Taizhou, Jiangsu, 225300 Phone: 86-523-86201352 Fax: 86-523-86816890 Web: https://www.cwbio.com E-mail: [email protected] EC-Representative SUNGO Europe B.V. VAT: NL857821659B01 Add: Olympisch Stadion 24, 1076DE Amsterdam, Netherlands Contact: SUNGO Secretary Tel /Fax: +31 (0) 2021 11106 E-mail: [email protected] Version: A/2 Effective Date: 2020-08-05 Magbead Viral DNA/RNA Kit Product: Magbead Viral DNA/RNA Kit Size: 96 tests/Box, 800 tests/Box Applications It is used for nucleic acids isolation, enrichment and purification. The processed products can be used for clinical diagnosis in vitro. Principle Magbead Viral DNA/RNA Kit provides a simple, rapid and efficient method to extract DNA/RNA from swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids. The unique buffer system enables the nucleic acid in the lysate to be efficiently and specifically binded to the magbeads. The obtained nucleic acid has high purity, stable quality, and is free of protein, nuclease and other contaminants and inhibitors. It can be applied to various conventional operations, including PCR, fluorescence quantitative PCR and other experiments. CoWin Biotech CoWin Biotech

Storage Condition and Valid Period Store at 4-30°C up to 12 months. We suggest products transportation at 4-37°C for no more than 7 days. Sample Requirements Applicable Samples: swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids. Equipment and Reagents to Be Supplied by User 1) Constant temperature mixer (CWBIO, CW2593 is recommended) 2) 2/15ml Magnetic device (CWBIO, CW2594 is recommended) 2. Automated extraction (Compatible with CWBIO's automated nucleic acid extractor, CWE2100) 1) Automated nucleic acid extractor (CWE2100) 3. Automated extraction (Compatible...

3. Compatible with CWBIO's automated nucleic acid extractor CW9600. DNA / RNA can be extracted from 96 samples at one time using CWE9600. 3.1 General purification procedure 3.1.1 Add the reagents to each well of the 96 DW plate according to the table below (the sample needs to be balanced to room temperature). 3.1.2 Put the plates into CW9600 according to equipment tips, and run CWE9600 program. After 20 minutes, take out the Plates, and the samples of elution plate were transferred to the centrifuge tube for long-term preservation at -80°C. 3.2 Rapid purification procedure (swab sample only)...

1.1.8 Let it dry for 2-5 minutes to remove residual ethanol. 1.1.9 Add 40-100 |jl RNase-Free water to the centrifuge tube, vortex for short seconds, and shake 5 minutes with a constant temperature mixer of 1200 rpm at 56 C. 1.1.10 Keep the centrifuge tube on the magnetic device, after the magbeads are absorbed, transfer the nucleic acid solution into a new centrifuge tube, and store it at -80°C for prolonged preservation. 1.2 Rapid purification procedure (swab sample only) 1.2.1 Take 1.5 ml centrifugal tube (supplied by user), add 300 pl sample (the sample needs to be balanced to room temperature),...