Background

This document provides information on a nucleic acid test kit designed for the simultaneous detection of three respiratory pathogens: SARS-CoV-2 (COVID-19), Influenza virus, and Respiratory Syncytial Virus (RSV). These viruses cause acute respiratory infections with overlapping symptoms such as fever, fatigue, and cough, making differential diagnosis important.

Pathogen Overview

• COVID-19 (SARS-CoV-2): An acute respiratory infectious disease with high susceptibility among people. Asymptomatic carriers can transmit the virus. Main symptoms include fever, fatigue, and dry cough.
• Influenza Virus: Highly infectious pathogen causing acute respiratory infections with rapid spread, high morbidity, and some mortality.
• Respiratory Syncytial Virus (RSV): Commonly infects children worldwide, causing bronchiolitis and sometimes severe complications like pneumonia and respiratory failure, especially in young children and the elderly.

Test Kit Description

The test kit uses fluorescent probe-based real-time PCR technology to detect specific gene targets from the three pathogens in respiratory samples. It allows simultaneous detection in a single reaction tube, improving efficiency and enabling early diagnosis.

Key Features

• Detection Targets: ORF1ab gene for SARS-CoV-2, M1 or NS1 gene for Influenza virus, and F gene for RSV.
• Specimen Types: Oropharyngeal swabs, sputum, and bronchoalveolar lavage fluid.
• Kit Size: 50 tests per kit.
• Compatible Equipment: Applied Biosystems 7500, Stratagene MX 3000P, BioRad CFX96, and other real-time PCR systems capable of detecting four channels simultaneously.

Performance Advantages

• High Efficiency: Simultaneous detection of three pathogens in one tube.
• High Sensitivity: Detection limits are 100 copies for SARS-CoV-2, 400 copies for Influenza virus, and 200 copies for RSV.
• High Specificity: No cross-reactivity with other pathogens, ensuring reliable results.
• Internal Control: Included to monitor the experimental process and ensure test validity.

Clinical Significance

Early and accurate identification of infections caused by SARS-CoV-2, Influenza virus, and RSV enables timely isolation and treatment, helping to prevent transmission and manage outbreaks effectively. Since symptoms overlap among these viruses, this test aids in differential diagnosis to guide appropriate clinical decisions.

Manufacturer Information

Jiangsu Mole Bioscience Co., Ltd.
Addresses:
- 6-7th Floor, G116 Building, No.805 Jiankang Avenue, Medical New & Hi-tech District, Taizhou, Jiangsu Province, China. Tel: 0523-86080606
- 4-5th Floor, Building No.5, Health Valley, No.1500 Wenyi Xi Road, Yuhang District, Hangzhou, Zhejiang Province, China. Tel: 0571-87209306 / 87209308
Stock code: 839185