PRODUCT BULLETIN ClarityTM Digital PCR System With digital PCR this fast and easy, the choice is now clear. Adopting an innovative tube strip design, the Clarity™ system brings digital PCR analysis to the next level by significantly reducing its preparation time and simplifying its workflow. Capable of running parallel reactions, more samples can now be analysed in a day to provide unsurpassed digital PCR throughput. It is now possible to do more with less – run more The ClarityTM Digital PCR Reader reactions in shorter time with less effort. Unique chip-in-a-tube design simplifies cumbersome workflow typically associated with digital PCR Performs up to 96 digital PCR reactions per experiment Well-suited for high throughput analysis CHIP-BASED PARTITIONING Stable with minimal sample loss CLOSE TUBE FORMAT Prevents cross contamination among reactions Compatible with conventional thermal cyclers Optimised for both TaqMan® probe-based and EvaGreen® dye-based assays Unrivalled system and sample cost

System Workflow The ClarityTM system adopts a unique chip-ina-tube design which allows digital PCR to be performed with ease and speed. The workflow begins with preparation of the reaction mixture, which can either be TaqMan® probeor EvaGreen® dye-based. Each reaction mixture is partitioned into 10,000 reactions by a high-density chip within each tube. After partitioning, the reactions are subjected to thermal cycling, followed by partition detection using the ClarityTM Reader. By analysing the number of partitions that are positive or negative for PCR products, the DNA copy number is then determined...

Figure 2. Plotted graphs of the measured concentration of RNase P against its expected concentration for the TaqMan® probe-based (left) and EvaGreen® dye-based (right) assays. Both assays showed excellent linearity (R2>0.999) across a dynamic range of over 4 orders of magnitude. The lower limit of detection was found to be <1 copy per microliter of reaction. Error bars correspond to the standard deviation of each data set. Find out more on our website: www.jnmedsys.com Need technical support? Contact our scientists: [email protected] For Research Use Only. Not for use in diagnostic procedures....