For the L arg Anim e al Veter inari an. SQUEEZETEST® Modified Wisconsin fecal egg per gram count This technique using the SqueezeTest kit will help provide a quantitative count of the parasite loads in eggs per gram. 1. Mark the outside of the SqueezeTest with client information. A - Attached spoon B - Collection container and mixing chamber C - Cone-shaped sealed cap D - Built in mesh filter 2. Collect 2-3 grams of fresh fecal material by using the attached spoon on the side of the SqueezeTest. A gram of feces is roughly the size of a marble. This estimate will get a sample of within ±10% of weighing it. 3. Remove SqueezeTest cap and deposit sample and spoon inside. Then put the cap back on to transport it back to the clinic. 4. Cut off ¼” of SqueezeTest tip cap with a nail trimmer (J84h). 5. Using a wash bottle (J818) filled with flotation solution (Sheather's Sugar Solution J1028g) add more or less 20mls of solution and keep the container tilted, then add solution slowly to prevent air lock. Remember that LESS IS MORE when adding fecal solution because if you add too much solution to the test, you will be throwing away diagnostic ova. If you did not fill the centrifuge tube, you may top it off with fresh solution before centrifuging.

6. Mix the solution with fecal matter by gently squeezing the SqueezeTest bottom. Be careful to avoid spillage. 7. Fill test tube (J546a) completely and create a meniscus right in the swing arm centrifuge, place coverslip and spin. By using a swing arm centrifuge there is no waiting period after the centrifuge stops.The filter in the SqueezeTest will remove most extraneous matter. One can also use the SqueezeTest without a centrifuge, but yields will be less than if done with a centrifuge. When not using a centrifuge one should not use Sheathers solution. They should use Sodium Nitrate or Zinc...