Manual version：A-2502EN nano-material reflect fluorescence signal, and the strength of the signal is positively Feline Coronavirus Antibody (FCoV -Ab) Test Kit Instruction correlated with the concentration of FCoV IgG in the sample. （Rare Earth Nano-Fluorescence Immunochromatography) 【Main ingredients】 1. FCoV Ab test reagent card Please read the instructions carefully and follow the instructions before use. This product is only for in vitro veterinary diagnosis 【Product name】Feline Coronavirus Antibody Test Kit (FCoV- Ab one step test) 【Packing specifications】 10 tests/ box 【Preservation conditions and expiry date】 【Detection purpose】 Feline coronavirus (FCoV) infection is very common in cats. It is generally believed that Storage at room temperature (RT) with an effective period of 36 months; the virus can cause diarrhea and infectious peritonitis. When the cat was infected with After the test card is opened, it must be used within 20 minutes. 【Applicable models】 FCoV, the body would produce FCoV antibody. The antibody content in serum and ascites of cats with typical symptoms of infectious peritonitis was much higher than that of cats 【Sample requirements】 with intestinal infection caused by common FCoV. If a high level of antibody is detected in Whole blood (or whole anticoagulant blood) : for immediate use; the blood or ascites of a sick cat with suspected symptoms of infectious peritonitis, it will Serum/plasma: the whole blood should be centrifuged as soon as possible. If the indicate that the cat has a high possibility of suffering from infectious peritonitis. obtained serum/plasma cannot be timely detected, it can be stored at 2~8℃ for 2 days Antibody detection has a certain significance of diagnosing negative. If the antibody in the Ascites: if the ascites can not be detected in time, it can be stored at 2~8 ℃ for 2 days. blood is very low in the first day, and the antibody is not significantly increased after 7 【Precautions】 1. This product is only for in vitro diagnosis by veterinarians, and it is strictly prohibited days of monitoring. The possibility of infectious peritonitis can be ruled out. Clinical significance: 1) Quantitative monitoring of FCoV antibody concentration to determine whether the cat is 2. This product must be used within the validity period. 3. When used, the test card and sample diluent should be restored to room temperature. 2) The detection of high FCoV antibody indicates that the possibility of infectious 4. Different batches and products are not allowed to be mixed. 5. Before the experiment, the standard curve of the corresponding batchs must be 3) Negative diagnosis of infectious peritonitis was made. 【Detection principle】 6. The test card shall be inserted into the instrument within 20s after the sample is added. FCoV IgG antibody content in cat blood was detected by fluorescence 7. This product cannot be used as the basis for the final diagnosis of immunity, and immunochromatography. The basic principle is as follows: There are T lines and C lines on should be combined with other diagnoses for comprehensive judgment. the nitrate fiber membrane. The binding pad is sprayed with fluorescent nanomaterial 8. Try to avoid using hemolytic whole blood samples. marker that can specifically recognize FCoV IgG. FCoV IgG in the sample first binds to 9. Each test card has one sample diluent, and it is strictly prohibited to reuse different the marker to form a complex, which then binds to the T-li

[Operating procedures] 1. Turn on the instrument,, and enter the information of the test case. 2. The sample and sample diluent should be restored to room temperature; 3. Pipette draw 5 pL of whole blood/ascite (or 2.5 pL serum/plasma) into the sample diluent and mix well; 4. Open the small cover (White) on the top of the dropper, inverted and squeeze the dropper to drop the mixed liquid (3 drops=100 pL) into the sample hole of the test card; 5. After adding the sample, insert card into the slot within 20s, and click ” Standard Test ”; Or after adding the sample, the test card should be inserted...