Manual version：A-2502EN 1. FCV Ab test reagent card Feline Calicivirus Antibody（FCV-Ab）Test Kit Instruction （Rare Earth Nano-Fluorescence Immunochromatography) Please read the instructions carefully and follow the instructions before use. This product is only for in vitro veterinary diagnosis 【Preservation conditions and expiry date】 【Product name】Feline Calicivirus Antibody Test Kit Storage at room temperature (RT) with an effective period of 36 months; After the test card is opened, it must be used within 20 minutes. 【Applicable models】 (FCV Ab one step test) 【Packing specifications】 10 tests/ box 【Detection purpose】 Feline calicivirus (FCV) infection is a feline viral respiratory diseases. The 【Sample requirements】 general clinical manifestations are mainly upper respiratory symptoms, namely Serum/plasma: Whole blood needs to be separated as soon as possible. If the obtained depression, serous and mucous rhinorrhea, conjunctivitis, stomatitis, tracheitis, bronchitis, and biphasic fever. FCV infection is a frequently-occurring disease in serum/plasma cannot be tested in time, it can be stored at 2~8 °C for 2 days. 【Precautions】 cats, with high morbidity and low mortality. The content of FCV IgG antibody in a 1. This product is only for in vitro diagnosis by veterinarians, and it is strictly prohibited cat can reflect the immune status of the body. Clinical significance: 2. This product must be used within the validity period. 1) For the evaluation of the body before vaccination; 3. When used, the test card and sample diluent should be restored to room temperature. 2) Detection of the body's antibody titer after vaccination; 4. Different batches and products are not allowed to be mixed. 3) Early detection and diagnosis during the period of FPV infection. 5. Before the experiment, the standard curve of the corresponding batchs must be imported. 6. The test card shall be inserted into the instrument within 20s after the sample is added. 【Detection principle】 7. This product cannot be used as the basis for the final diagnosis of immunity, and should be combined with other diagnoses for comprehensive judgment. This product uses fluorescence immunochromatography to quantitatively detect the content of FPV IgG antibody in cat blood. The basic principle is as follows: 8. Try to avoid using hemolytic whole blood samples. There are T lines and C lines on the nitrate fiber membrane. The binding pad is 9. Each test card has one sample diluent, and it is strictly prohibited to reuse different sprayed with fluorescent nanomaterial marker that can specifically recognize FCV IgG. FCV IgG antibody in the sample first binds to the marker to form a complex, which then binds to the T-line. Under the excitation light, nano-material reflect 【Operating procedures】 fluorescence signal, and the strength of the signal is positively correlated with the

1. Turn on the instrumented enter the information of the test case. 2. The sample and sample diluent should be restored to room temperature; 3. Pipette draw 10 p L of serum/plasma into the sampl e diluent an d mix well; 4. Open the small cover (White) on the top of the dropper, inverted and squeeze the dropper to drop the mixed liquid (3 drops=100 pL) into the sample hole of the test card; 5. After adding the sample, insert card into the slot within 20s, and click ” Standard Test ”; Or after adding the sample, the test card should be inserted into the card slot after 10 minutes of timing response,...