Catalog No. : J1115 Manual version: A-2502EN Feline Immunodeficiency Virus Antibody(FIV-Ab) Test Kit Instruction (Rare Earth Nano-Fluorescence Immunochromatography) Please read the instructions carefully and follow the instructions before use. This product is only for in vitro veterinary diagnosis [Product name] Feline Immunodeficiency Virus Antibody Test Kit (FIV Ab one step test) [ Packing specifications ] 10 tests/ box [ Detection purpose ] Feline Acquired Immune Deficiency Syndrome(AIDS) is a disease caused by feline immunodeficiency virus ( FIV ) in cats . In terms of structure and nucleotide sequence, it is related to the HIV that causes AIDS in humans. It also often produces clinical symptoms of immunodeficiency similar to human AIDS, but FIV in cats is not transmissible to humans. Therefore, reliable and effective detection has a positive guiding role in prevention, diagnosis and treatment. [ Detection principle ] The product uses fluorescence immunochromatography to quantitatively detect the FIV Ab content in cat serum/plasma. The basic principle is as follows: There are T line and C line on the nitrate fiber membrane. The T line is a secondary antibody that specifically recognizes cat IgG. The binding pad is sprayed with fluorescent nanomaterial marker that can specifically recognize FIV IgG. FIV IgG in the sample first binds to the marker to form a complex, which then binds to the T-line. Under the excitation light, nano-material reflect fluorescence signal, and the strength of the signal is positively correlated with the concentration of FIV IgG in the sample. [Main ingredients] 1. FIV Ab detection reagent card [Preservation conditions and expiry date] Storage at room temperature (RT) with an effective period of 36 months; The test card shall be used within 20min after it is opened. [Applicable models] [Sample requirements] Serum/plasma: Whole blood needs to be separated as soon as possible. If the obtained serum/plasma cannot be tested in time, it can be stored at 2~8°C for 2 days. [Precautions] 1. This product is only for in vitro diagnosis by veterinarians, and it is strictly prohibited to use it in humans. 2. This product must be used within the validity period. 3. When used, the test card and sample diluent should be restored to room temperature. 4. Different batches and products are not allowed to be mixed. 5. Before the experiment, the standard curve of the corresponding batchs must be imported. 6. The test card shall be inserted into the instrument within 20 seconds after the sample is added. 7. This product cannot be used as the basis for the final diagnosi s of immunity, and sho uld be combined with oth er diagnoses for comprehensive judgment. 8. Try to avoid u sing hemolytic whole bl ood samples. 9. Each test card has one sample diluent, and it is strictly prohibited to reuse different samples. [ Operating procedures ]

1. Turn on the instrumented enter the information of the test case. 2. The sample and sample diluent should be restored to room temperature; 3. Pipette draw10 pL of serum/plasma into the sample diluent and mix well; 4. Open the small cover (White) on the top of the dropper, inverted and squeeze the dropper to drop the mixed liquid (3 drops=100 pL) into the sample hole of the test card; 5. After adding the sample, insert card into the slot within 20s, and click ” Standard Test ”; Or after adding the sample, the test card should be inserted into the card slot after 10 minutes of timing response,...