Feline Pancreas Lipase (fPL) Test Kit Instruction (Rare Earth Nano-Fluorescence Immunochromatography) Please read the instructions carefully and follow the instructions before use. 【Preservation conditions and expiry date】 This product is only for in vitro veterinary diagnosis Store at room temperature (RT), with a validity of 36 months. 【Product name】Feline Pancreas Lipase Test Kit ( fPL one step test) After the test card is opened, it must be used within 20 minutes. 【Applicable models】 Applicable to JZ001 for testing. 【Detection purpose】 Feline pancreatitis is an inflammatory infiltrating disease of the pancreas. Generally can 【Sample requirements】 Serum/plasma: Whole blood needs to be centrifuged as soon as possible. If the obtained serum/plasma cannot be detected in time, it can be stored at 2~8 °C for 2 days. 【Precautions】 be divided into acute pancreatitis and chronic pancreatitis. In acute pancreatitis, pancreatic neutrophil infiltration, pancreatic necrosis, fat necrosis around the pancreas, edema and injury can be seen. In chronic pancreatitis, pancreatic fibrosis and atrophy can be seen. Compared with acute pancreatitis, chronic pancreatitis is less harmful, but more frequent. 1. This product is for in vitro diagnostic use only by veterinarians, and cross-use from human is strictly prohibited. 2. This product must be used within the validity period. 3. When using, the test card and sample dilution should be returned to room temperature. 4. Cross-mixing of different batches and different products is strictly prohibited. 5. Before the experiment, the standard curve of the corresponding batchs must be imported.. 6. The test card should be inserted into the instrument to start the test within 20s of dropping the sample. 7. This product cannot be used as the basis for the final diagnosis of the disease, and it needs to be comprehensively judged in combination with other diagnosis. 8. Try to avoid using hemolyzed whole blood samples. 9. Each test card corresponds to a sample dilution solution. Reuse of different samples is strictly prohibited. When a cat suffers from pancreatitis, the pancreas is damaged, and the cat pancreas lipase content in the blood increases sharply. At present, pancreatic lipase is one of the most specific indicators for diagnosing cat pancreatitis 【Detection principle】 This product uses fluorescence immunochromatography to quantitatively detect the content of fPL in whole blood, serum/plasma. The basic principle is as follows: There are T lines and C lines on the nitrate fiber membrane. The T-line is coated with an antibody A that can specifically recognize the fPL. On the binding pad is another antibody B, fluorescently labeled with a nanomaterial, which also binds to fPL. fPL in the sample and antibody B form a combination and then chromatography. The complex reaches the T-line and binds to antibody A to form a sandwich structure. Under the excitation light, nano-material reflect fluorescence signal, and the strength of the signal is positively correlated with the concentration of fPL in the sample. 【Main ingredients】 1. fPL detection reagent card

1. Turn on the instrument, and enter the information of the test case. 2. The sample and sample diluent should be restored to room temperature; 3. Pipette draw 20 p L of serum/plasma to the sampl e diluent an d mix well; 4. Open the small cover (White) on the top of the dropper, inverted and squeeze the dropper to drop the mixed liquid (3 drops=100 pL) into the sample hole of the test card; 5. After adding the sample, insert card into the slot within 20s, and click "Standard Test "; Or after adding the sample, the test card should be inserted into the card slot after 10 minutes of timing response,...