Group: ATTOPLEX
Catalog excerpts
Attoplex® SARS-CoV-2 Real-time PCR Kit 사용목적 Use Intended Mini Kit, QIAGEN and MagNa Pure 96, Roche), and the extraction This product is an in vitro diagnostic medical device that qualitatively method follows the instructions for each extraction kit. detects a genetic material of COVID-19 (SARS-CoV-2) extracted from the nasopharynx and oropharynx of patients suspected of respiratory infection using Real-time RT-PCR (real-time reverse transcriptase 2.1 After thawing the frozen component completely on Ice, spin- polymerase chain reaction). The kit includes RT-qPCR Master Mix and Primer & Probe Mix, which can specifically amplify ORF1ab region 1, ORF1ab region 2, and N gene of SARS-CoV-2 variants, and provide positive and negative control to evaluate the validity of the test results. Kit Components 제품의 구성 Components 2X One-step RT- Colorless solution Light purple solution Probe Mix Positive Control in a brown tube Colorless solution in a clear tube if it is placed at room temperature for more than 1 hour or continuous freezing and thawing. 3.1 Extract the RNA sample from the specimen using a commercially available RNA extraction kit. 3.2 Prepare the reaction solution according to the table below. 필요한 장비 및 기구 Required Material and Devices • Applied Biosystems™ 7500 Fast Real-time PCR Instrument system or CFX96™ Real-Time PCR Detection System • Vortex mixer • Table-top Centrifuge • Pipettes and Pipette tips with aerosol barrier • Disposable powder-free gloves Procedure 사용방법 ※ Warnings and Precautions Always wear protective disposable powder-free gloves when handling kit components. Volume (μL/rxn) 2X One-step RT-qPCR Master Mix Sample (or Positive/Negative control) *Packing unit: 100 tests/Kit ※ It should be noted that the product performance will be affected 3) Reaction and PCR Setup Volume Colorless solution down to collect the solution to the bottom of the tube. 3.3 Close the lid of the tube, vortexing lightly, spin-down to collect the solution to the bottom of the tube. ※ If air bubbles remain in the tube, remove them as they may affect the real-time PCR reaction. ※ After preparing the reaction solution in which all components are mixed, real-time PCR is performed immediately. 3.4 After setting the real-time PCR program according to the PCR conditions and fluorescence channels of the table below, the amplification proceeds. Step Avoid microbial and nuclease (DNase/RNase) contamination of Always use DNase/RNase-free disposable pipette tips with aerosol barriers. • Discard sample and assay waste according to your local safety regulations. 1) Sample Preparation 1.1 The sample to be used in this diagnostic product is RNA extracted from specimens collected from the nasopharynx and oropharynx of humans. 1.2 For the extraction of RNA from a sample, it is recommended to use a commercially available extraction kit (eg, QIAamp Viral RNA
Open the catalog to page 1General Precautions 사용시 주의사항 4) Data Analysis 4.1 Set Threshold and Baseline values according to the table below. Threshold ORF1ab region 1 ORF1ab region 2 N gene other purposes. CFX96 Auto fitting* Auto fitting* Auto Auto Auto fitting* Auto fitting* *Setting → Baseline setting → Baseline Subtracted Curve Fit and Apply Fluorescence Drift Correction • It should be used by skilled experts, and be sure to follow the instruction for use. • Be sure to follow the instructions when handling samples and kits. • Use separated and segregated working areas for (i) sample preparation, (ii) reaction...
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