Enterovirus Test Kit-IFU
2Pages

{{requestButtons}}

Catalog excerpts

Enterovirus Test Kit-IFU - 1

Attoplex® Enterovirus Real-time PCR Kit method follows the instructions for each extraction kit. 사용목적 Use Intended This product is an in vitro diagnostic medical device that qualitatively detects a genetic material of Enterovirus extracted from the respiratory and stool, cerebrospinal fluid (CSF), and blood samples of 2.1 After thawing the frozen component completely on Ice, spin- patients suspected of Enterovirus infection using Real-time PCR (realtime polymerase chain reaction). The kit includes qPCR Master Mix and Primer & Probe Mix, which can specifically amplify target gene of Enterovirus, and provide positive and negative control to evaluate the validity of the test results. Colorless solution Light purple solution Probe Mix Positive Control in a brown tube Colorless solution in a clear tube continuous freezing and thawing. available RNA extraction kit. 3.2 Prepare the reaction solution according to the table below. Volume (μL/rxn) 2X One-step RT-qPCR Master Mix Sample (or Positive/Negative control) Colorless solution 3.1 Extract the RNA sample from the specimen using a commercially Volume *Packing unit: 100 tests/Kit 필요한 장비 및 기구 Required Material and Devices • Applied Biosystems™ 7500 Fast Real-time PCR Instrument system or CFX96™ Real-Time PCR Detection System • Vortex mixer • Table-top Centrifuge • Pipettes and Pipette tips with aerosol barrier • Disposable powder-free gloves Procedure 사용방법 ※ Warnings and Precautions • if it is placed at room temperature for more than 1 hour or down to collect the solution to the bottom of the tube. ※ It should be noted that the product performance will be affected Always wear protective disposable powder-free gloves when 3.3 Close the lid of the tube, vortexing lightly, spin-down to collect the solution to the bottom of the tube. ※ If air bubbles remain in the tube, remove them as they may affect the real-time PCR reaction. ※ After preparing the reaction solution in which all components are mixed, real-time PCR is performed immediately. 3.4 After setting the real-time PCR program according to the PCR conditions and fluorescence channels of the table below, the amplification proceeds. Step Avoid microbial and nuclease (DNase/RNase) contamination of Always use DNase/RNase-free disposable pipette tips with aerosol barriers. • Discard sample and assay waste according to your local safety regulations. 1) Sample Preparation 1.1 The sample to be used in this diagnostic product is RNA extracted from specimens collected from the respiratory and stool, cerebrospinal fluid (CSF), and blood samples of humans. 1.2 For the extraction of RNA from a sample, it is recommended to use a commercially available extraction kit (eg, QIAamp Viral RNA Mini Kit, QIAGEN and MagNa Pure 96, Roche), and the extraction APX-IFU-RPT009/R01 Reference Dye (ROX) *IPC: Internal positive control 4) Data Analysis 4.1 Set Threshold and Baseline values according to the table belo

Open the catalog to page 1
Enterovirus Test Kit-IFU - 2

fitting* Auto fitting* *Setting → Baseline setting → Baseline Subtracted Curve Fit and 보관 Storage 및 취급방법 Protect all Kit components from light. Components Apply Fluorescence Drift Correction 4.2 Based on the Cut-off Ct value in the table below, the positive or negative of each target gene is determined. Positive (+) *U.D: Undetermined (or N/A: Not Applicable) **The Ct value of IPC is determined as a result of the negative control. Positive Control before after Nuclease-free water Expired 6 months after production 3 months 6 months after production 3 months 6 months after production 3 months...

Open the catalog to page 2

All ATTOPLEX catalogs and technical brochures