Viral NA Extraction Kit-IFU
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Attoplex® Viral NA extraction Kit 사용목적 Use Intended Attoplex Viral NA extraction kit provides easy and rapid method for culture medium, plasma, or serum, swab, urine, or virus-infected Always use DNase/RNase-free disposable pipette tips with aerosol barriers. can be directly used in various downstream applications, such as PCR, • RT-PCR, without any further manipulations. Avoid microbial and nuclease (DNase/RNase) contamination of the specimens and the components of the kit. liquid samples. Pure nucleic acids can be obtained in just 15 minutes without any use of hazardous organic solvent. Purified RNA and DNA Always wear protective disposable powder-free gloves when handling kit components. simultaneous isolation of pure RNA and DNA from cell-free fluid, cell Discard sample and assay waste according to your local safety regulations. Nuclease-free Water Mini column and Collecting tube • 1.1 Buffer VB, RW1 and RW2 are provided as concentrate. Absolute ethanol (ACS grade or better) should be added as below, before first use. All components of this kit should be stored at room temperature (15~25°C). Long exposure to heat source can deteriorate the During shipment or storage under cold ambient condition, a precipitate can be formed in buffer VL, VB(conc.) and/or RW1(conc.). Heat the bottle at 20~40°C to dissolve completely in such a case. buffer, dissolve completely at 20~40°C before use. 2) Procedure for purification of DNA/RNA from viral sample Buffer VB, RW1 and RW2 are provided as concentrate. Ethanol 1. Transfer up to 300 uL of sample into a 1.5 or 2 mL micro tube. must be added before first use as the indication on the bottle ◆ A sample can be used as forms of swab-storage media, cell-free Although the spin column can be stored at room temperature, it is ideal to store under cool ambient condition or in a refrigerator for prolonged conservation. Heat sources and direct sunlight must be avoided Required Material and Devices 필요한 장비 및 기구 • Absolute ethanol (ACS grade or better) • Pipettes and Pipettes tips with aerosol barrier • Vortex mixer • Table-top Centrifuge • Disposable powder-free gloves • 1.5 or 2 mL Microcentrifuge tube fluid, cell culture media, plasma, serum, urine, or other body fluid. ◆ When the sample is less than 300 uL, adjust the volume to 300 uL with 1x PBS or reduce the volume of the buffer VL and VB proportionally. 2. Add 500 uL of buffer VL to the tube and lyse the sample by pipetting or vortexing. ◆ It is critical for proper lysis to make the mixture homogenized. 3. Incubate the lysate for 10 mins at room temperature. ◆ After incubation, briefly centrifuge the tube to remove drops from the inside of the lid. 4. Add 700 uL of buffer VB to the lysate and mix thoroughly by vortexing or inverting. ◆ Do NOT cen

5. Transfer up to 750 uL of the mixture into a spin column, centrifuge for 30 secs at 13,000 xg, discard the pass-through, Trouble shooting guide 보관 및 취급방법 Facts Recommendations Use more sample. Concentrate the larger sample 6. If there is a remaining mixture, repeat the step 5 with them. Possible causes Low viral titer in and insert the column back into the collection tube. Poor quality of starting material 7. Apply 500 uL of buffer RW1 into the column and centrifuge for 30 secs at 13,000 xg. thawing of sample should be avoided. For proper lysis, it is essential to get homogenate by mixing...