Note:  For your own safety, read the instructions and follow the warnings and safety information on the device and in the instructions. Keep the instructions for future reference. Note:  In case you require this instruction in another language, please submit your request including the corresponding document number via e-mail or fax to KNAUER. Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland...

Table of contents 1. General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.1 About these instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 Signal words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.3 Additional typographical conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.4 Legal information . . . . ....

4.3 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 4.4 Connecting the leak management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 4.5 Inserting the flow cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 4.5.1 Inserting the flow cell with fiber optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 4.6 Connecting the capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

7.2 Operation Qualification (OQ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 8. Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 8.1 LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 8.2 Possible problems and solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 8.3 System messages . . . . . . . . . . . . . . . . . . ....

13. Reorders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 13.1 Devices and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 13.2 Flow cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 13.3 Flow cells with fiber optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 13.4 Fiber optic cables . . . . . ....

About these instructions These operating instructions enable the safe and efficient operation of the device. The user must have carefully read and understood these operating instructions before starting any work. The basic prerequisite for safe operation is compliance with all safety instructions (see „2 Basic safety instructions“, p. 3). In addition to the safety and warning instructions in these operating instructions, the local accident prevention regulations and the national industrial safety regulations apply. These operating instructions are an integral part of the device. It must be kept...

Legal information 1.4.1 Liability limitation The manufacturer is not liable for the following issues: Non-compliance of these instructions Non-observance of necessary safety precautions Improper use Operation of the device by unqualified personnel (see „2.2 User qualification“, p. 3) Use of non-approved spare parts Technical changes by the user such as opening the device and unauthorized modifications Violations of General Terms and Conditions (GTC) 1.4.2 Transport damage The packaging of our devices provides the best possible protection against transport damage. However, check the packaging...

2. Basic safety instructions The device has been developed and constructed in such a way that hazards arising from its intended use are largely excluded. Nevertheless, the following safety instructions must be observed in order to exclude residual hazards. Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail. 2.1.1 Operating ranges The device is intended to be used indoors for chromatographic applications. 2.1.2 Foreseeable misuse Refrain from the use of the device for the following purposes...

Basic safety instructions Operator responsibility The operator is any person who operates the device himself or leaves it to a third party for use and who bears the legal product responsibility for the protection of the user or third parties during operation. The obligations of the operator are listed below: Know and follow the applicable work safety regulations Identify hazards arising from the working conditions at the place of use in a risk assessment. Set up operating instructions for the operation of the device. Regularly check whether the operating instructions correspond to the current...

Basic safety instructions Working with solvents 2.6.1 General requirements The user is trained for handling different solvents. Note recommended solvents and concentrations in these instructions in order to avoid personal injury or damage to the device. For example, certain chemicals may cause PEEK capillaries to swell or burst (see „14 Chemical compatibility of wetted materials“, p. 71). Note that organic solvents are toxic above a certain concentration. For handling hazardous solvents see the following section. Mobile phases and samples may contain volatile or combustible solvents. Avoid the...