FeedBeads

FeedBeads

FeedBeads

Product catalog summary
Introduction
FeedBeads are polymer-based particles designed for controlled glucose delivery in bioreactors, facilitating substrate-limited fed-batch processes without the need for additional equipment like tubing or pumps. This technology is particularly beneficial for high throughput screening (HTS) and improves the reproducibility and synchronization of pre-cultures.
Specifications
  • Available in two sizes: Ø 12mm for shake flasks and Ø 6mm for microtiter plates.
  • Glucose is the primary substrate, with other crystalline nutrients available upon request.
Procedures
  • FeedBeads enable fed-batch operations in shake flasks or microtiter plates, allowing for additional screening routines under substrate-limited conditions.
  • Conditions for use include a 0.1M phosphate buffer at pH 7 and a temperature of 37°C.
Benefits
  • Improves screening security and is suitable for HTS.
  • Reduces overflow mechanisms and avoids issues like the Crabtree Effect and Overflow Metabolism.
  • Facilitates smooth scale-up and increases the probability of identifying optimal production strains.
Applications
  • Used in YFP production with recombinant E. coli under specific conditions (mineral medium, 37°C, shaking diameter 50mm, shaker speed 350 rpm).
  • Fed-batch preculture of E. coli BL21 YFP in MTP using Wilms-MOPS medium without a carbon source.
Order Information
  • SMFB63319: FeedBeads Glucose (Ø 12mm), pack of 25.
  • SMFB63318: FeedBeads Glucose (Ø 6mm), pack of 100.
Development and Support
The Slow Release technology was developed by the University of Technology, Aachen, supported by Deutsche Stiftung Umwelt. It is represented by Adolf Kühner AG in Switzerland.
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Catalog excerpts

FeedBeads-1

Polymer based slow release system

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FeedBeads-2

Release Kinetic of the ø 6mm FeedBeads Conditions: 0.1M phosphate buffer, pH = 7, T = 37°C 2 Release Kinetic of the ø 12mm FeedBeads Conditions: 0.1M phosphate buffer, pH = 7, T = 37°C FeedBeads Controlled glucose delivery by slow release technology FeedBeads provide substrate limited fed-batch conditions in the shaken bioreactor without the need for enzymes or additional equipment such as tubing or pumps. + Improves screening security + Suitable for high throughput + + + + + + screening (HTS) Easy handling Fed-batch without tubing and pumps Reduces overflow mechanism of the culture Reproducible...

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FeedBeads-3

YFP-Production with recombinant E. coli Mineral medium; temperature 37°C; shaking diameter 50mm; shaker speed 350 rpm; VL = 12.5 mL FeedBeads | 3 Fed-Batch preculture of E.coli BL21 YFP in MTP Wilms-MOPS medium without carbon source; temperature 37°C; shaking diameter 50mm; shaker speed 400 rpm; VL = 700 µL Product information 25 pieces, Ø12mm (shake flasks), 100 pieces, Ø 6mm (microtiter plate) • Substrate glucose on request other crystalline nutrients are available as the limited substrate Order information: SMFB63319: FeedBeads Glucose (Ø 12mm), pack of 25 SMFB63318: FeedBeads Glucose (Ø 6...

 Open the catalog to page 3
FeedBeads-4

Fed-Batch without tubing & pumps www.kuhner.com Adolf Kühner AG • since 1949 Dinkelbergstrasse 1 CH – 4127 Birsfelden ( Basel ) Switzerland

 Open the catalog to page 4

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.