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SB10-X

Product catalog summary

Overview
The SB10-X is an orbital shaken benchtop bioreactor designed for cultivating human, mammalian, and plant cells in single-use bags. It is suitable for research, process development, and pilot scale applications, with a working volume range of 4 to 12 liters.

Key Features

Fast and simple setup with single-use bags, eliminating the need for cleaning and sterilizing.
Online measurement and control of pH and dissolved oxygen (DO) using built-in optical sensors.
Efficient liquid mixing through orbital motion, reducing mechanical stress on cells.
Robust Kuhner direct drive for precise control of orbital speed.

Modules and Components

Vessel Module: User-friendly design, easily detachable for transport.
Filter Heater: Prevents water vapor condensation, ensuring smooth operation.
Control Unit: Features a 12” touch screen, USB and Ethernet connections, and Kuhner Insight software for data management.
Pumping System: Includes three peristaltic pumps for aseptic fluid management.
Gas Mixing Device (FlowCon): Allows precise gas mixing with four mass flow controllers.

Technical Specifications

Shaking Speed: 20 to 140 rpm with adjustable shaking diameter.
Temperature Control: Heating resistance with a maximum of 40°C.
pH and DO Measurement: Optical chemosensors with high accuracy and low drift.
Gas Mixing: Supports up to four gases with programmable pressure limits.

Accessories

Single-use Bags: Available in standard, basic, and perfusion types, each with specific features for different applications.
Light Protection Jacket: Protects sensitive media from light exposure.
MagFix: Magnetic tube holders for secure tubing management.

Compliance and Options

GMP Compliance: 21 CFR Part 11 compliant software.
IQ-OQ Documentation: Available for GMP procedures.
Additional Interfaces: EMI-60 and NET-60 for enhanced connectivity.

Catalog excerpts

r Kuhner shaker SB10-X (OrbShake) Orbital shaken benchtop bioreactor for single-use bags

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The SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It consists of six modules (excluding the bag) and has a working volume from 4 litres up to 12 litres. The SB10-X can be used in research, process development or as a pilot scale bioreactor. Performance characteristics + Cultivation of human, mammalian and plant cells + Fast, simple set up + Easy scale-up + Online measurement and control of pH and DO + Three different single-use bags available Single-use bag for a fast and simple set up The cylindrical vessel...

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SB10-X (OrbShake) | 3 ᕣ Filter heater Trouble free scale-up The SB10-X fills a gap in the scale-up chain from commonly used single-use flasks all the way up to 50 L and 200 L bioreactors. The consistent hydrodynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range. The culture from the SB10-X can serve as a preculture for the SB50-X and SB200-X OrbShakers. The SB10-X can also be used as a pilot scale bioreactor. Heating and online pH & DO measurement ᕡ The tray module, which is fixed onto the ES-X shaker module, contains integrated heating and the...

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Dimensions (mm) Control unit Dimensions (mm) SB10-X Shaken bioreactor Technical data • SB10-X Order number: SMX7600 includes 6 modules: - Shaker module ES-X Bioreactor: - Tray module - Vessel module - Cable and tube management module - Filter heater - Control unit • Shaken bioreactor Working volume 4 L - 12 L Total bag volume approx. 24 L Weight (bioreactor incl. shaker) approx. 75 kg without liquid Required footprint (excl. control unit) 669 mm x 646 mm • Shaker module ES-X Motor modified ES-X benchtop shaker Shaking diameter 50 mm (orbital motion) shaking diameter is adjustable (12.5 mm, 25...

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Casing Stainless steel 1.4301 front is splash proof and dust protected (IP 64) Resolution 1024 x 768 Cooling passive with no fan Main memory 2 GB (1.6 GB usable) Monitoring pH & DO, shaking speed, temperature, gas flow and mixture, pressure Calibration pre-calibrated sensors in bag Recalibration one point recalibration is possible Supervisor Supervisor Tube 1.6 mm wall thickness Technical data subject to change

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0 Gas mixing: iMixing up to four gases iGas mixtures can also be connected Gas mixing device (FlowCon) 0 Number of mass flow controllers Ambient conditions Input Pressure measurement Differential pressure up to 4 gases (air, oxygen, nitrogen, carbon dioxide) different gas mixtures can also be connected push in connector for tubes OD 6 mm required input pressure: 1 bar - 2 bar 1 (tube fitting optimal for tubing 9 x 1 mm) Flow rates: N2: 0.1 L min-1 - 1.99 L min-1 accuracy: ± 1.3 % of full scale gas output: pressure sensor DELOS SI (Jumo), integrated in FlowCon 0 mbar - 100 mbar (programmable)...

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ᕦ Light protection jacket Accessories Single-use bag Kuhner offers three different single-use bag types to meet all user needs. Each pack contains three bags. These gamma-irradiated bags for the Kuhner shaken bioreactor require no additional mixing device, enable quick set up times and eliminate elaborate cleaning and sterilising procedures. All bags have a working volume from 4 litres up to 12 litres. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The standard bag (SMX760001) has built in optical sensors for pH and DO which allow non-invasive online measurement...

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