L-Hydro
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Sxe the ate 1960S 2 'V-nbc* st Ticthods have been developed of proposed and used lor p'eservafon ol tissue products- These include soiutons containing alcohol high ionc strength of high osmolarity. Most of these methods aim at p'evenlirg the protein components in tissues from s^oslances osed may inhibit subsequent chemical treatment methods or cause complications in SLOsequent processes.

Glutaradchyde as tissue preservation has Lee- usee lo' over 40 years, and ■ beer sixcesstul in treat ng different botogCa xenograft tissues used in heart vaVes Glutaradehyde's main (unction is lo establish crossJirk connectons in the collagen [hat can provide structure and Collagen Strand Reactive Groups

1 ■ Glutaraldehyde reouces imrnunogenicity: ii is one the main reasons for the success ot this method ol peservatoa such as n giutaradehyde-treateo xenograft r SSJCS tor bbp'osthetic heal valves.3 2- An exce ent slerila^l: One musl remember this when working with a non-g Paraldehyde method 0" treating implanted tissues. 3- Reativety duraole: The durability oJ g jtaraldenyde treated issue valves demonstrated by in vitro fatigue tester, was party resoonsibte lor the successful development o( the clinical glularaldefiyde treated porcine 1 - Cytotoxic: li has been demonstrated that...

Many publications have shewn thai gk/tarakJehyde is sM a good option tor tissue treatment, as $ obtains good results for old patients in duratMy and resistance. problem for this technology, for many years methods to reduce calcification have been developed. None of them 100% calcium free, proof that this feature is not well seen in congenital defects repair

So What is the Goal in Tissue Treatment? • Establish biological tissue • Inhibit antigenicity • Reduce I Suppress the inflammatory response • Guarantee sterility • Enable cell reconstruction after implant

L-Hydro Principles 1. Present (o receptor cells a non viable xenograft matrix (acelular), similar to natural human matrix. 2. Cells grow in same natural function 3. Reconstruction stimulated by similar natural matrix and physiologic conditions In Vivo - tissue engineering." Based or these principles. Labcor in collaboration with Dr. David Cheung, PhD, Heart Institute of Montana5 have developed this method of tissue treatment technology, by the name L-Hydro. Preserve xenog-aft products to be implanted in hLmans as potentially capaole to functonaly reconstruct with no rejection and/or...

L-Hydro Tissue Treatment Howitworks Sails ETOH Mild Chemical Oxidation Fresh tssue ts harves:ed TOTH the submerged n a cc*d etharal salts soluticn - Bto Ixrden redxtioa Ptrform mid chemical oxoaxn rraix;rxj pate'itml cxid/aoJe mate^ls that can attract ritiarnmaEcry 'oacticn f'OTi :fic fios: Awcii-kiown psty-giyco w^ch bras tig'iiy to colagen is also added to mask the potential nor- cxractcd anbgoa

Anti-Inflammatory Agent Anani-nflammaioiyagcni sacteoo to 'edu<^ ir'LTTTTiuHlofy rt-;-ip::rse Dung \no rplant procedure a hecann ccatng rodjeos eel Mild Chemical exact properties ol tfee

Inflammatory Cells Endothelial Cells 'issue Colls

Tissue Matrix inflammatory cells, endothelial cells are permitted to grow. This way endothelial cells allows infiltration of connective cells becoming the synthesis

• PolymcnTation roacton • Coating. Cytotoxic ghJtaalOef-iyoe • Vfcxtficalon of MUno Groups • Break down of toxc poyne«s ■ °e&$ton\ resduaJ arcgeneoTy • ^erasta"! esdual cytotoxicity Conventional - depends on Toxicity of giutaiakJohyo© mprovod TILJIII stop reduction of boburdon, Inon Iral sto- zatior 1 Aia&yde slorage/shipping solulion * Coating, nor Cytotoxic poty gfycoi • No froaftcalior. o* ATWIO Grcxx)S • No txeak down of towc poymers • No persistent re$<|jaJ artgeneoly • No parstslent resdual cytotoxicity • Mull* step reduction of C*oburden - L-Hyc»o process • Final liquid prttse...

Endothefial Cells

• Tissue Growth - New Tissue Matrix • No persistent residuai antigenicity • Very iow level of calcification • Preserve natural structural and mechanical characteristics Poicine pulmora-y vave

Biological tissue treatments • Heart valves Labcor Products Treated with L-Hydro • Babygraft / Corograft / Perigraft (Mesenteric artery) (Pericardium conduit, portion ctosecf with porcine pulmonary valve) Qfobed jugular vein) (Pericardium repair membrane)

1 - Wtlans, Meivyn A>, ER.C.S.: Tissue Vaves in Young Patients - A recipe tor Disaster. Department Of Cardio thorasic sygery. Provincial HosoitaL Pen E zabeth, South Africa Journal ol Carcfcac Surgeiy Vol 6, No 4, 2 - Jameson, W. R trie e: a). : Carpen:ier tdwards Sandard Porcioe Biophfosthe&s: A 21 -years Experience, University of British Cclurrkba. Vancojvor, Canada- Ann 3 - Salgaler M, L, Bajpai R K.; Immunogenicity ol glu:araklchyOo-l'C3tcd bovho ooricaraal tissue xonograf:s in rabbrls. Unheralty ol Dayton, Daylon. Ohio. Journal of Biomedical Materials Research, 1985; 19:1-12 4 - Nina...