Metabolomics Analysis

The Established Leader in GC-MS Metabolomics Metabolomics presents challenges for both the analytical methods used and the data reduction required to interpret the results. No single analytical technique can be used for complete characterisation of the metabolome, and no metabolome has been completely characterised. However, GC- MS provides an established method to analyse the primary metabolome, whereas LC investigates the secondary and tertiary metabolites. As the established leader in GC-MS metabolomics1– 4, LECO products deliver the accuracy, resolving power, deconvolution, and speed to characterise...

® PEGASUS  BT GC-TOFMS A proven quantitative and qualitative workhorse, the Pegasus GC-TOFMS is a staple of leading metabolomics laboratories. The high sensitivity, peak capacity, and reproducibility of GC combined with Time-of-Flight Mass Spectrometry (TOFMS), provides benefits such as reduced analysis times, industryleading peak deconvolution, and an ability to re-interrogate rich data sets repeatedly for biological inference. ® LECO’s innovative StayClean  Ion Source design virtually eliminates the need to clean your source – lowering downtime and enhancing productivity. Furthermore, the metabolomics...

® PEGASUS  BT 4D GC×GC-TOFMS: See What Else is in Your Sample A pioneer in comprehensive two-dimensional gas chromatography, LECO empowers metabolomics researchers worldwide looking for answers to the question, “What else is in my sample?” The example below shows how the second dimension of chromatography separates 1D coelution very clearly. The complexity of biological samples often presents one of the biggest challenges to increasing our knowledge. Having the availability of increased separation space allows the scientist to unlock more information from the samples without the possible bias...

GC×GC-TOFMS Sensitivity Advantage In addition to the increased identification capabilities and peak capacity, cryogenic focusing occurs at the modulator to sharpen the peaks prior to detection with MS. This enhanced peak detectability is illustrated with arachidonic acid, trimethylsilyl ester, shown below in overlaid one-dimensional and two-dimensional data. Less abundant analytes are enhanced significantly, typically analytes which are below the detection limit with one-dimensional chromatography. These benefits combine to offer a more complete picture of a sample relative to GC alone. Proven...

® High Resolution GC-MS: the PEGASUS  GC-HRT + uncertainty in identification. Accurate mass data reduces the number of potential molecular formula for ions and enables more effective interpretation of mass spectra. Plus, the combination chemical ionization (HR-CI) with electron impact (HR-EI) ionization, which both create accurate mass fragments, provides a proven mechanism for molecular ion identification. This results in more confident interpretation of biochemical pathways, particularly when analytes are differentially expressed. With unknown metabolites presenting one of the most significant...

Confirm Your EI Library Match with Soft Ionization As universal and data rich as HR-EI is, there can still be ambiguity as to molecular ion identification. High Resolution Chemical Ionization (HR-CI) can provide a solution to this problem by delivering a softer ionization mechanism for preservation of the molecular ion, which can then be used to formulate a calculation in its own right, and to filter the possible library hits provided by the EI spectral library search from seven possible identifications to one clear known. Library Match 679/1000 Anhydro-sorbitol (TMS) = C18 H44 O5 Si4 Win the...

LECO’s Commitment to Quality and Service Every day around the world, LECO instruments continuously perform analyses for today‘s most complex applications. Whether you are analysing samples in the food, flavour / fragrance, petroleum, environmental, or life-science (metabolomics) industries, we have an instrument configuration to meet your needs. Our global network of sales and support includes over 25 subsidiaries worldwide, and is dedicated to customer service and satisfaction. Our commitment to quality is further underscored with ISO-9001:2015 certification. We conform to CE quality and safety...