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DFC9000

Product catalog summary

Introduction
The document introduces the Leica DFC9000, a monochrome microscope camera designed to capture live cells in near-native conditions using advanced scientific CMOS (sCMOS) sensor technology. This camera is particularly suited for fluorescence imaging, allowing for the capture of faint signals and dynamic processes.

Key Features

Short Exposure Times: Protects cells by minimizing exposure.
High-Speed Acquisition: Captures dynamic processes efficiently.
Large Field of View: Provides more information in a single image.

Specifications

Camera Type: 4.2 MP sCMOS monochrome fluorescence camera.
Sensor: Front-illuminated scientific CMOS CIS2020A from BAE.
Shutter: Rolling shutter with global reset.
Pixel Size: 2048 x 2048 (4.2 MP); 6.5 μm x 6.5 μm.
Sensor Size: 13.3 x 13.3 mm (~19 mm diagonal).
Acquisition Speed: 50 fps (USB 3.0); >90 fps (Camera Link).
Quantum Efficiency: ~82% @ 580 nm.
Bit-depth: 12 bit / 16 bit.
Cooling: 0°C @ 27°C ambient, air-cooled.
Binning: Options include 2x2, 3x3, 4x4, 8x8.
Partial Scan: Freely definable region of interest (ROI), combinable with binning.
Internal Memory: 1 GB.
Dark Current: ~0.14 e-/px/sec.
Read Noise: 0.9 e-.
Dynamic Range: 1:33000.
Pixel Clocking Rate: 540 MHz / 216 MHz.
Supported Operating Systems: Windows 7 and Windows 8.
Software: Leica Application Suite (LAS) X.
Interfaces: USB 3.0 or Camera Link.
Camera Package: Includes camera head, cables, PCIe board, LAS X DVD, and instructions.

Quantum Efficiency vs. Fluorophore Emissions
The document includes a graph showing the quantum efficiency of the camera across different wavelengths, highlighting its performance with various fluorophores such as CFP, GFP, Alexa 594, and others.

Conclusion
The Leica DFC9000 is a powerful tool for researchers needing high-quality fluorescence imaging, offering advanced features that enhance imaging capabilities while protecting live cells.

Catalog excerpts

Leica DFC9000 GT Leica DFC9000 GTC sCMOS Microscope Camera Get Closer to Reality Imagine you could image live cells as close as possible to their native state. Capture even faint fluorescent signal you currently miss. And discover what happens in the time between two subsequent images. Imagine you could get a better view of what really happens in cells! The Leica DFC9000 will turn your imagination into reality. This monochrome microscope camera with newest scientific CMOS (sCMOS) sensor technology for fluorescence imaging enables you to image your cells under near-native conditions. • Protect your cells with short exposure times • Capture highly dynamic processes with high-speed acquisition • Get more information in one image with large field of v

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SPECIFICATIONS Camera type 4.2 MP sCMOS monochrome fluorescence camera Copyright © by Leica Microsystems CMS GmbH, Wetzlar, Germany, 2016. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

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