HIGH QUALITY ULTRAMICROTOME FOR PRECISE ROOM TEMPERATURE AND CRYO SECTIONING Leica EM UC7 / Leica EM FC7

HIGHEST QUALITY SECTIONING High quality sectioning of specimens for light, electron, and atomic force microscopy examination has never been easier and more precise. Leica Microsystems introduces its latest specimen preparation technology: the Leica EM UC7 ultramicrotome and the Leica EM FC7 cryo chamber attachment. Leica Design by Werner Höl

The Leica EM UC7 prepares excellent quality semi- and ultra-thin sections, as well as the perfectly smooth surfaces required for LM, TEM, SEM, and AFM examination. The precision mechanics, ergo omic design, and n intuitive layout of the touchscreen control unit make the Leica EM UC7 ideal for the highest quality specimen preparation. >> ucentric movement of the stereomicroE scope observation system with patented designated positions for specimen approach, for glass and diamond knives. >> n addition to the standard LED illumiI nation for top light and backlight and transmitted light, the LED spot...

ERGONOMIC DESIGN Comfortable to use Leica Microsystems designed the Leica EM UC7 / FC7 with specific features that make it a user-friendly, ergonomic instrument providing comfort and minimizing strain for each user. Easy, fast adjustments adapt the instrument to accommodate multiple users. Fatigue-free operation is an integral benefit of the Leica EM UC7 / FC7 design. The Leica M80 stereomicroscope with ErgoWedge accessory adapts the height and angle of the optical head to adjust perfectly to an individual’s body height and position. Arm rests are attached to the instrument table. These features,...

OPTIMAL POSITIONING OF THE OPTICAL HEAD The Eucentric Movement of the Leica EM UC7 viewing system allows examination of sections, even with a lowered water level e.g. for Lowycryls and dry sections. For accurate approach of the knife towards the specimen with the backlight, the viewing angle must be set according to the type of knife in use. The patented defined position marks of the eucentric movement provide maximum approach accuracy either for glass or diamond knife approach. Section observation with lowered water level without eucentrically moveable stereomicroscope. Section observation with...

Top: Knife edge with particles visible under spot light illumination. Bottom: Site-specific trimming with spot illumination. ADVANCED FEATURES Motorized knife stage Motorized North-South movement of the knife stage is a unique feature of Leica ultramicrotomes. The additional implementation of motorized East-West movement of the Leica EM UC6 was a logical step forward. Additionally, the E-W quick adjustment buttons on the Leica UC7 controller allow efficient traversing of the knife. Motorization of the stage has also allowed many useful features to develop hand in hand with it, for example the...

Easily activated anti-static function The Leica EM CRION ionizer dissipates electrostatic charging that can occur at a specimen’s surface, minimizing static effects associated with climatic changes and the sectioning of certain materials. This adjustable antistatic electrode is a useful accessory for sectioning at room temperature. Adjusting the ionizer output is an integral function of the Leica EM UC7 controller; no additional controller is required on the instrument table. The simple press of a footswitch synchronizes and activates the simultaneous operation of the specimen arm motor and the...

EASY-TO-USE TOUCHSCREEN CONTROL All functions of the Leica EM UC7, the Leica EM FC7 cryo chamber and the Leica EM CRION are controlled via touchscreen. A choice of two touchscreen control units is available to operate the ultramicrotome cryo chamber and ionizer. 7” basic touchscreen control unit Indentations in the control panel casing enable the user to locate routinely-used controls that are adjusted while observing the specimen. While viewing the specimen, the user can set the cutting window and step advance and perform many other functions

Advanced Control The innovative advanced touchscreen control unit of the UC7 enables fast and safe alignment of knife and specimen with help files and prompts to hand for beginners Programmable knife and cutting sequences offer significant advantages for automated trimming (AutoTrim). The outstanding performance of the touchscreen is reflected in its ability to control all necessary components for sectioning. These include the built-in antistatic Leica EM CRION device control, the Leica EM UC7 cryo chamber control, storage of up to one hundred different user, specimen, and knife parameters and...

Counter window to toggle between section counter and feed totalizer, countdown, E-W measuring, and AutoTrim mode. Leica EM FC7 control field appears as soon as the chamber is connected. As soon as the Leica EM CRION ionizer is connected and switched on this window appears to adjust the intensity of the ionizer. To optimize diamond knife usage, up to 100 different knives can be stored. Each knife is divided into a number of segments. The selected segment is approached automatically with the motorized knife stage (patent applied). As soon as the upload button is pressed the grid box window appears...

LEICA EM FC7 Within minutes the Leica EM UC7 can be equipped with the Leica EM FC7 low temperature sectioning system, which incorporates many features and offers a wide range of user benefits such as: > The Leica EM FC7 and Leica EM CRION ionizer controls are both integrated with the Leica EM UC7 control unit to provide easier operation and more workspace. > Using the advanced touchscreen control, three different cryo-modes are available: - Standard - High gas flow - increased GN2 gas flow reduces ice contamination below -140 °C - Wet sectioning - to set a temperature difference of up to 130...

ADVANCED CRYO-SECTION COLLECTION Leica EM CRION ionizer and micromanipulator Especially for frozen hydrated sectioning or the Tokuyasu method, the Leica EM CRION with electrostatic discharge and charge mode used with the micromanipulator provides outstanding cryo sectioning performance. The micromanipulator easily attaches to the Leica EM FC7 cryo chamber, which allows the grid to be exactly positioned close to the knife edge using the micrometer gauges. Once these positions are defined, fast retraction of the grid can be performed manually prior to sectioning to prevent possible influence of...