Leica AF6000 Modular Wideeld Systems for Advanced Live Cell Research

Live Cell Imaging Solutions that Grow with Research Needs Ease of use • All experiment settings visible at a glance • Full screen live image display • Fast and intuitive setup of experiments • Context sensitive online help • Minimum training required Biological and medical researchers are probing deeper into the secrets of life. Wideeld uorescence microscopy is a key technology for imaging living cells. Developmental and molecular processes of cells can be visualized directly by wideeld uorescence techniques shedding new light on the undiscovered. Leica Microsystems’ advanced wideeld systems...

Application examples: High-speed time-lapse experiments, see page 6 Developmental biology, see page 6 Studies of live cells up to whole organisms Macro time-lapse experiments, see page 6 In vivo imaging Cell cycle studies Axon outgrowth Intracellular transport, vesicle trafcking Exocytosis, endocytosis Calcium imaging, Fura2, see page 8 FRET ratio and FRET sensitized emission, see page 8 Dendritic cells infected with bacteria Green: GFP expressing enteropathogenic E. coli Red membrane staining: Cy3 labeled anti Cd44 antibody Courtesy of Nadzeya Kramko, ZMBE, Institute of Infectiology, University...

A System as Dynamic as Modern Research The Leica AF6000 systems offer full exibility to adapt to your changing needs. You will benet from the utmost reliability and convenience, regardless of the modules and components you select to congure an individual system. As many of the system components are designed and developed by Leica Microsystems, we offer unparalleled automation control due to complete integration of our hardware within the software platform. Add a control unit to convert the system into a high-speed live cell imaging workstation with full real-time control. Leica Microsystems’...

Leica AF6000 Software Modules Acquisition modules • Basic software module for all Leica AF6000 modular systems • Microscope control • Processing tools • Full image viewer • Full review capability • Quantification tools • Driver for compatible Leica DFC cameras • Configuration tool for Leica AF6000 systems Multi Channel Acquisition Experiments with several fluorescence channels Z-stack acquisition includes software based autofocus Define duration and frequency of image capture for time-lapse experiments, see page 6 Mark & Find Multi positioning experiments, see page 7 Acquire mosaic images Create...

Leica AF6000 Time-Lapse Applications Superior live cell imaging • Stay in focus with hardware-based Adaptive Focus Control (AFC) • Software-based autofocus • Brilliant images under physiological conditions with dedicated 63x live cell objective • High-speed acquisition • Extensive range of live cell accessories 2D and 3D time-lapse experiments over several hours or even days require the highest level of stability, reproducibility, acquisition speed and system reliability. With the AF6000, Leica Microsystems offers an integrated modular system solution to meet these requirements. Leica microscope...

Stitching Experiments The chances of observing a process of interest are much higher when several locations are observed. With the Mark & Find function, an unlimited number of user defined sample positions can be defined. This allows recording of many different cells within one time-lapse experiment, thus saving precious time and resources. At each stage position a cell specific Z-stack size can be defined. With the Pause and Relocate button, every single Mark & Find position can be corrected in its X, Y and Z location during the running experiment. The corrections can also be Such tools are...

Get Instant Feedback with the Calcium and FRET SE Modules Dedicated analysis modules • FRET sensitized emission and FRET ratio • Subtraction of background fluorescence • Stay in control of your experiment at any - online generation of measurement graph - online display of ratiometric image - online generation of image statistics Cultured hippocampal astrocytes from 18-day-old embryos of Sprague-Dawley rats (Objective lens: Plan APO 20x0.7, 4 Ratio/sec) Courtesy of Drs. Kazunori Kanemaru and Masamitsu lino, Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Japan...

The Ultimate in Flexibility and Automation Dene acquisition macros with the Live Data Mode The Live Data Mode module is designed to dene acquisition jobs and macros within the easy-to-use interface of Leica AF6000. Within the macros, loops can be dened, a pause can be added and up to four trigger IN channels and four trigger OUT channels can be set. An Event Manager shows the start point of each job or macro. User dened events can also be generated during the experiment and can be edited online to add comments. The Live Data Mode module is therefore the ideal accompaniment to the Calcium Imaging...

"Cell Biology is a fascinating field of research with a wide range of applications. My equipment needs to grow with the increasing num- just like the Leica Microsystems imaging solutions." University of Marburg, Department of Clinical Marburg, Germany Leica AM TIRF MC at a glance: • Integration of four wavelengths with fast AOTF control • Correction of the penetration depth when switching wavelength • Combination of TIRF and fast FRET analysis • Highest transmission of all optical TIRF components for maximum TIRF imaging speed and brilliance • Fast image recording of up to 30 fps with simultaneous...

y – TIRF and Super-resolution The Leica SR GSD offers the highest lateral resolution with uorescence wideeld and TIRF microscopy. It is a superb tool for determining the structure of uorescently labeled samples in neurobiology, cell biology, virology and microbiology. The Leica SR GSD provides detailed insights into cellular structures and molecular sociology that cannot be seen with diffraction limited light microscopy. Using ground state depletion technology (GSDIM), it is possible to control the emission of uorochromes and localize the position of single molecules down to a precision of 20...