Technical Documentation

Redefine the Limits of Microscopy Widefield super-resolution with ground state depletion ≡≡ ≡≡ ≡≡ ≡≡ ≡≡ ≡≡ ≡≡ 2 Maximum resolution down to 20 nm The SuMo Stage, with Suppressed Motion technology, minimizes drift for accurate localization of molecules Online super-resolution image projection – see results as they are acquired F ull application flexibility offered by combining super-resolution with TIRF and epifluorescence on a multi-purpose live cell imaging system Standard fluorochromes can be used – no need to change protocols Powerful lasers for the highest flexibility in fluorochrome selection...

Technical Specifications Hardware Microscope stand Leica DMI6000 B / AM TIRF MC Sample stage SuMo Stage 11888963 Objective (GSD) HCX PL APO 100x/1.47 Oil CORR TIRF PIFOC Objectives (additional) • • • • • Right sideport obligatory: used for camera Andor iXon3 897 Left sideport optional for additional camera Bottom port not supported • • • • • Mounted at SuMo stage Needed for GSD super-resolution imaging Special stage for GSD super-resolution Different stages for standard applications can be exchanged by customer DIC prism pairs for GSD systems Mounted to nosepiece with 7.5 mm adapter ring Lasers:...

0 Inverted research microscope Leica DM16000 B 0 Active anti vibration table O Anti vibration table 0 Leica EL6000 external light source Additional objectives Order number

1405 mm 750mm Distance from wall 600 mm 2600 mm Distance from wall 600 mm 1500 mm Maximum footprint of the Leica SR GSD with antivibration table, laser rack and system desk. Installation Requirements Environment Room temperature: +15 to + 30°C Air condition required, but avoid proximity to GSD system Protect from dust Room darkening recommended Humidity max 80%, non condensing Temperature stability ±1°C (±1.8°F) ; ΔT<0.5°C/15min Electricity supply The system requires two mutually independent fuse circuits. One fuse circuit for the main system and the second for the laser rack. 2x Supply voltage...

Leica SR GSD Cameras 11 600 220 Keyboard US 11 600 240 Andor iXon DU-897 EM CCD back illuminated 11 600 245 Trigger cable Andor cameras 11 600 221 Keyboard UK * Peripheral triggering 2 channel in and 1 out 11 640 004 Adapter peripheral triggering 11 547 004 Leica DFC365 FX 11 600 243 Trigger cable DFC Cameras 11 600 223 Keyboard Italian 11 600 224 Keyboard German 11 547 003 Leica DFC345 FX 11 600 243 Trigger cable DFC Cameras 11 541 510 1.0x C-mount adapter 11 600 225 Keyboard Spanish 11 600 222 Keyboard Swedish 11 547 002 Leica DFC310 FX 11 600 243 Trigger cable DFC Cameras 11 600 219 Keyboard...

Optional stages for system used as imaging platform (exchange of GSD stage with following stages possible) 4 channel in and 4 out 11 640 004 Adapter peripheral triggering 11 640 002 BNC-Box 11 522 078 Regular xed stage plate 11 522 012 Fixed heating stage plate 11 522 013 Fixed cooling stage plate 11 522 014 Objective guide for special holder frame Special holder frames Stage Inserts 11 532 536 Scanning Stage H117 N1 DM (requires High-Speed Board 6500, not compatible with Water Immersion Dispenser) Fast FIM 11 504 129 FFIM 11 600 234 Universal mounting frame Pecon inserts and CO2 Covers 11 504...

“With the user, for the user” Leica Microsystems • Life Science Division The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems’ customers at the leading edge of science. • Industry Division The Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative...