Leica TCS SP8 L IF E SCIENCE RESE A RCH DI V ISION Looking Forward to Your Discoveries

Leica TCS SP8 - Looking Forward to Your Discoveries 4 From Whole Organisms to Highest Level of Detail 6 Focus on Your Research - Automation & Reliability Redefined with Leica TCS SP8 14 Leica Microsystems Innovation Synergies 16 Ready to Grow - Future-proof with the Leica TCS SP8 18 Leica HyD™ - All-purpose Super-Sensitivity 20 Leica Confocal Objectives - The Best Objectives for Superior Images 22 LAS AF 3 - Intuitive Software that Makes Your Life Easier 24 Leica TCS SP8 X - Freely Tunable Excitation 26 Leica TCS SP8 STED - Opening the Gate to Super-Resolution 28 Leica TCS SP8 MP - Look Deeper...

LEICA TCS SP8 – LOOKING FORWARD TO YOUR DISCOVERIES 5 Looking Forward to Your Discoveries From routine imaging to live cell research, from super-sensitivity to super-resolution, from multiphoton imaging to CARS – whatever your research, Leica Microsystems has the confocal for your application. SENSITIVITY BY DESIGN FASTER FOR NEW BIOLOGY READY TO GROW The design of a confocal microscope Biological research is advancing at an As new scientific approaches unfold, so requires excellent separation of ever increasing rate as is the range of too do the instrumentation requirements excitation and emission...

6 From Whole Organisms to Highest Level of Detail 2 Large field – whole organism. By using an X2Y scan mirror architecture, Leica confocal systems support the largest field of view in point scanning. You can take images of whole animals or plants, often without stitching. See your specimens in widescreen.

LEICA TCS SP8 – SUBCHAPTER 2 7 3 Mosaicking (stitching) is available for samples larger than the field of view. Save time on stitching by covering the same area with a smaller number of images. Cover more ground, while preserving even the finest detail.

8 Leica TCS SP8 – Innovations at a Glance Leica Confocal Scanner FOV (Field Of View) scanner Tandem Scanner › See the full specimen in one shot (save time on stitching) (FOV and resonant scanner combined) › Increased time resolution › High-speed FRAP experiments with soluble proteins › Higher throughput in screening experiments › Improved cell viability › Rapid 3D particle tracking Galvo flow (3D stacking without inertia) Leica HyD™ (Hybrid photodetector) › Vibration-free, continuous movement › Reduced light dosage improves cell viability › Real-time XZ-slices › Ideal for high-speed imaging ›...

LEICA TCS SP8 – INNOVATIONS AT A GL ANCE 9 Intermediate optics LIAchroics: AOBS: Acousto-optical beam splitter Low incident angle dichroic beam splitters › Programmable beam splitter provides the highest flexibility › High suppression of excitation light for high contrast › Support of White Light Laser (WLL) › Custom-designed low incident angle dichroics › Completely transparent for unhindered spectroscopy › Cost-efficient beam splitting › Steep cut-off for maximum photon efficiency › Longer sample viability with low power of excitation laser › Switching in microseconds for fast multicolor kinetics...

10 4 Color-coded maximum intensity projection of Zebrafish muscle. Acquired using second harmonic generation (SHG) signal excited by IR laser.

LEICA TCS SP8 – SENSITIVIT Y BY DESIGN 11 Sensitivity by Design Designing a high precision optical instrument such as a confocal microscope is the ultimate balancing act of excellent beam splitting, high scan speed, and flexibility. At the same time, every photon emitted by the sample needs to be preserved so it can contribute to your brilliant imagery. PRE-PROGRAMMED FLEXIBILITY A STRONG TEAM COUNT ON IT For coupling a wide range of excitation Efficient routing of fluorescence light Leica‘s digitally sampled photomultiplier lasers into the confocal scanner, Leica between excitation and detection...

TANDEM SCANNER-TIGHT SPEED CONTROL Leica Tandem Scanners contain switchable galvanometric mirrors. The high-speed mirror resonates at either 8 or 12 kHz, which results in frame rates of more than 420 fps (at 512 x 16 scan format). This high speed is possible because of intricate real-time control using an internal frequency calibration system. In spite of the rapid scanning the image geometry is faithfully recorded. Auto Gain GALVO FLOW - RAPID Z-STACKING By continuously propelling the insert rather than in discrete steps, the system minimizes inertia. This avoids an otherwise rate limiting repeated...

LEICA TCS SP8 – FASTER FOR NEW BIOLOGY 13 Faster for New Biology Unveiling the processes of life is a never-ending challenge. Leica Microsystems combines innovative elements that amplify each others' performance to provide accelerated confocal image recording. Arrive at new discoveries with greater speed. A TANDEM FOR LIVE SPECIMENS SWEEP THROUGH LIFE BROADEN YOUR SCOPE The observation of rapid biological The unique z-stacking strategy of using a Some moments in life sciences are too processes requires high-speed imaging galvanometric stage insert provides precious to be missed, which is why...

14 Focus on Your Research Biological knowledge is expanding, but your time is not. Thankfully, technology can take away some of the pressure by automating the tedious and repetitive parts of experiments. Leica Microsystems can be your confocal partner, giving you the confidence to push your experimental limits and trust in your data. PEACE OF MIND WITH READY, SET, ... LIGHT! LEICA REMOTECARE For quantifiable results, you need both a › Save time managing your lab equipment reliable detection system and a stable › Safe and auditable light source. A much overlooked source › Pro-active maintenance...

16 Leica Microsystems Innovation Synergies PHOTONIC EXPLORATION SPECTRAL FREEDOM IMAGING The AOBS and second generation White Light Laser For multi-channel imaging, the Leica TCS SP8 (unique Lightgate, FLIM, pulse picker) complete the confocal prism-based spectral detection system most innovative light path concept in confocal optimizes the detection of your valuable sample’s imaging. Combine this with trillions of excitation emissions. Unlike diffraction grating and barrier combinations and the unique “lambda squared” scan, filter-based systems, it works at lower excitation and you are ready...