Dual Color STED 3 μm Dual Color Confocal Leica TCS SP8 STED – Opening the Gate to Super-Resolution Explore what lies beyond the diffraction barrier with Leica Microsystems‘ confocal super-resolution system. The Leica TCS SP8 STED provides fast, intuitive, and purely optical access to structural details far beyond the diffraction limit – even in living specimens. › Resolve details smaller than 50 nm – quickly and directly › Discover what‘s inside – with the only confocal super-resolution system › Watch dynamics in living cells – at the nanoscale! › Freedom to choose – use popular fluorescent dyes and proteins › Perform colocalization studies – with dual color STED imaging › Profit form sharper images at lower laser power – with gated STED › Allow your Leica TCS SP8 to grow – upgrade to STED www.leica-microsystems.com

Liprin GFP in drosophila larvae. Sample courtesy of Stephan Sigrist, Freie Universitat Berlin, Germany. LEICATCS SP8 STED-THE FAST TRACK TO SUPER-RESOLUTION STED microscopy does not rely on mathematics. What you see is whatyou get. Of course, results can be improved further by deconvolution. Just one click instantly switches from confocal to super-resolution, even during a live scan. Within seconds, imaging parameters can be optimized and super-resolved images obtained STED microscopy works with standard fluorophores such as Alexa 488, Oregon Green 488 and FITC. It does not rely on oxygen depletion...