Non-S1 phase S1 phase Cells expressing a cell cycle stage marker. Green: non-S1 phase. Red: S1 phase. Courtesy of Dr. Malte Wachsmuth and Dr. Lars Hufnagel, EMBL, Heidelberg, Germany Leica TCS SP8 X – The Only Freely Tunable Confocal System The white light laser source of the Leica TCS SP8 X perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Continuously tunable excitation and detection ensure best image quality and sample protection. Up to eight excitation lines can be used – simultaneously. › Tuning range of 470 to 670 nm in 1 nm intervals › Minimum cross-talk in multiple stained specimen › Change of Illumination regimes within microseconds › Lambda Square Mapping: full spectral information by excitationemission correlation › LightGate: background quenching using time-gated detection › True FLIM results by adjustable excitation wavelength and variable pulse repetition rate www.leica-microsystems.com

Specimen: fixed cells with triple staining: GalNacT2_GFP (golgi), LAMP-546 (endosomes), Calnexin 594 (ER). FLIM measurements at different excitation wavelength (A: 486 nm, B: 542 nm, C: 594 nm). Sample: courtesy of Matthias Weiss, Cellular Biophysics Group, Bioquant, Heidelberg, Germany. FREELY TUNABLE CONFOCAL IMAGING WITH THE LEICATCS SP8X The White Light Laser (WLL) is the optimal excitation source to image any kind of dye combination. The acousto-optical beam splitter (AOBS) is the key to selecting any wavelength from the white spectrum. Completed by the tunable spectral detector (SP), the...