TCS SPE
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Catalog excerpts

TCS SPE - 1

Simply Sophisticated - Your Personal Confocal

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TCS SPE - 3

Leica TCS SPE – Simply Sophisticated Many exciting discoveries begin with a confocal from Leica Microsystems. Here is one to start with – the Leica TCS SPE. Providing all the features needed for r outine confocal techniques, it offers excellent quality imaging at an affordable price. HIGH FIDELITY SPECTRAL IMAGING INTEGRATES ANYWHERE VERSATILE WORKHORSE The Leica TCS SPE is an affordable entry The Leica TCS SPE is compact and fits Scientists worldwide know the Leica to the Leica family of confocals. It in every laboratory. The system is TCS SPE’s proven reliability. Its many combines a...

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TCS SPE - 4

Drosophila embryo Green: Mesh (Alexa 488), a smooth septate-junction-specific protein Magenta: Kune (Cy3), a pleated septate-junction-specific protein Mesh is required for smooth septate junction formation and the paracellular diffusion barrier in the Drosophila midgut. Format: 1024x1024 pixels, Objective: 20x, Zoom: 1x, Frame avg: 3 Courtesy of Dr. Yasushi Izumi Division of Cell Biology, Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University

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TCS SPE - 6

Versatile Confocal ›› Control box ›› Images without crosstalk ›› Automated filter control ›› Quadruple beamsplitter – no filter change necessary ›› Parallax-free scanner Spectral Detection (SP Detector) ›› Tunable from 430 to 750 nm ›› Multicolor imaging without crosstalk ›› Gapless spectral imaging ›› Prism-based dispersion gives highest light efficiency ›› Free from photon waste compared to a diffraction grating Solid State Lasers ›› 405, 488, 532/561, 635 nm excitation ›› Optional 405 nm excitation ›› Supply unit with small footprint ›

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TCS SPE - 7

Advanced Correction System (ACS) Objectives ›› Perfect compensation of focus shift ›› Maximum transmission from 405 nm to infrared ›› Minimum of moving parts in the light path ›› CS objectives for imaging with visible light LAS X Software ›› Intuitive workflow ›› FRAP wizard ›› Live Data Mode controls live cell experiments ›› Additional software packages for 3D visualization, 2D and 3D analysis High Content Screening with Leica HCS A ›› Large scale mosaics ›› One-click templates for multiwell plates ›› Open data formats support OME-TIFF ›› Computer-aided microscopy interface with online...

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TCS SPE - 8

SP Detection – Gapless Photon Efficiency Affordable, but comprehensive – the Leica TCS SPE is the instrument for highly resolved images. The filter-free spectral detection technology ensures the highest light efficiency for gapless spectral imaging. The result: brilliant images containing a maximum signal per-fluorophore-ratio with perfectly separated dyes. A TRUE CONFOCAL SCANNER SPECTRAL DETECTOR Equipped with a true confocal point scanner, the Leica TCS SPE As in every Leica confocal microscope, the dispersive element delivers images of the highest resolution by scanning the in the Leica...

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TCS SPE - 9

Spectral detection module of the Leica TCS SPE Diffraction patterns for two colors after passing through a prism. In multi-spectral imaging spectral specificity is influenced by the ability to resolve two adjacent diffraction patterns. Separation of two adjacent patterns, such as caused by different colors for circular (left), hexagonal (middle) and square (right) geometries. A 45 ° rotated pinhole is ideal for prism-dispersed light. Leica ACS Technology: Perfect Colocolization ACS OBJECTIVES FOR OPTIMAL COLOCALIZATION With the unique Advanced Correction System (ACS) objectives, Leica...

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TCS SPE - 10

Keep Living Cells Alive Imaging living specimens is essential to understanding cell dynamics in detail. The Leica TCS SPE is an ideal instrument for fast live cell imaging at full resolution. Comprehensive climate accessories keep your cells alive and healthy, and intuitive software packages even allow photo manipulation experiments. ENVIRONMENTAL CONTROL Fitting incubators to a Leica microscope stand creates a healthy environment for your cells. Users gain full control of the experimental conditions with the LAS AF Environmental Control module. The logged environmental data can be...

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TCS SPE - 11

Fluorescence Recovery After Photobleaching – FRAP 1.1 FRAP experiment with Leica TCS SPE. Pre-bleach, bleach, and fluorescence recovery with quantification. HeLa cells expressing Glycosylphosphatidylinositol anchored protein (gpi) fused to YFP, which is localized in the plasma. Sample courtesy: Stefan Terjung, EMBL, Heidelberg, Germany Fuorescence Recovery Signal, normalized LIVE DATA MODE – TAKE CONTROL The Live Data Mode tool in LAS X is designed for live cell experiments and dynamic investigations: Scientists can define an experiment before data acquisition to carry out manipulations on...

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TCS SPE - 12

Only Three Steps Away From Your Confocal Image The Leica TCS SPE’s streamlined approach makes it accessible to every user, beginners and experts alike. With the LAS X control software, Leica Microsystems o ffers an interface, which shows all you need to know about your confocal on one screen. The Leica TCS SPE gets the job done. ONE, TWO, THREE … CONFOCAL SET-UP WITH A TWIST The LAS X control software guides users step by step through The optional control panel adds smart features to your confocal. data recording and evaluation. The workflow design helps you Smart Gain and Smart Offset, for...

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TCS SPE - 13

1: Set up lasers 2: Adjust detector 3: Acquire Image Intuitive LAS AF user interface MODULAR SOFTWARE PLATFORM In addition to basic image acquisition such as lambda scan, sequential scan, time series and z stacks, LAS X can extend your research with a range of “High precision, robust technique and easy-to-use software is what we have always looked for. Leica Microsystems' confocal will become our workhorse for routine research.“ additional software packages like FRAP imaging, 3D visualization and 2D or 3D analysis. Live Data Mode is a tool for the easy set-up of complex, interactive...

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