sILSBio LifeSpan BioSciences, Inc. Triglyceride Assay Kit (Colorimetric) LS-K302-200 (200 Tests) • Store at -20°C Introduction TRIGLYCERIDE, also known as TRIACYLTRIGLYCERIDE or TRIACYLGLYCERIDE, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. Simple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery. LSBio's triglyceride assay uses a single Working Reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample. Key Features • Sensitive and accurate. Use as little as 10 pL samples. Linear detection range 0.01 mmol/L to 1.0 mmol/L (0.88 mg/dL to 88.5 mg/dL) triglyceride. • Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Applications • Direct Assays: triglyceride in biological samples (e.g. serum and plasma). • Drug Discovery/Pharmacology: effects of drugs on triglyceride metabolism. Materials Not Supplied Pipetting devices, centrifuge tubes, clear flat bottom 96-well plates (e.g. Corning Costar) and plate reader. Storage The kit is shipped on ice. Store all kit components at -20 °C. LifeSpan BioSciences, Inc. • 2401 Fourth Avenue, Suite 900, Seattle, WA 98121 www.LSBio.com • (206) 464-1554 • [email protected]

sILSBio LifeSpan BioSciences, Inc. Triglyceride Assay Kit (Colorimetric) LS-K302-200 (200 Tests) • Store at -20°C Assay Procedure Note: SH-group containing reagents (e.g. mercaptoethanol, DTT) may interfere with this assay and should be avoided in sample preparation. 1. Equilibrate all components to room temperature. Keep thawed Lipase and Enzyme Mix in a refrigerator or on ice. Dilute Standard in distilled water as follows. Transfer 10 pL diluted standards into wells of a clear 96-well plate. Diluted standards can be used for future assays when stored refrigerated. Serum and plasma samples should...

Triglyceride Assay Kit (Colorimetric) LS-K302-200 (200 Tests) • Store at -20°C Sample Data sILSBio LifeSpan BioSciences, Inc. FOR RESEARCH USE ONLY! Not for use in humans. LifeSpan BioSciences, Inc. • 2401 Fourth Avenue, Suite 900, Seattle, WA 98121 www.LSBio.com • (206) 464-1554 • [email protected]