DeepLabel™

DeepLabel™

DeepLabel™

Product catalog summary
Features
  • Lower concentrations of antibodies required for effective staining.
  • Simple protocol with ready-to-use reagents.
  • Supports multiple rounds of multiplexed antibody labeling.
Applications

The DeepLabel™ Antibody Staining Kit is designed for use with tissues cleared using X-CLARITY™ Systems and Reagents. It is compatible with various imaging techniques, including light sheet microscopy, two-photon microscopy, and confocal microscopy.

Product Description

The DeepLabel™ Antibody Staining Kit includes non-toxic, ready-to-use reagents optimized for clarified tissues. It allows macromolecular probes to penetrate thick, protein-dense tissues efficiently, enabling site-specific binding at lower antibody concentrations. The kit provides a 2.6 times greater signal-to-background ratio compared to conventional methods, facilitating vibrant fluorescence imaging at subcellular resolution.

Procedure
  1. Permeabilization: DeepLabel™ Solution A is used for permeabilizing clarified tissues.
  2. Antibody Labeling: Enhances antibody binding for multiple rounds of labeling.
  3. RI Matching: X-CLARITY™ Mounting Solution is used for refractive index matching, reducing photobleaching and preserving fluorescence signals.
Performance

DeepLabel™ significantly enhances antibody penetration and site-specific binding in thick, cleared tissues, resulting in robust labeling with excellent signal-to-noise ratios. It is particularly effective in staining mouse brains, providing better antibody distribution and low background noise.

Ordering Information
  • DeepLabel™ Antibody Staining Kit: Cat # C33001
  • X-CLARITY™ Mounting Solution: Cat # C13101
  • Various packaging options available for different quantities.
Example Applications
  • Adult mouse brain stained with anti-β-Amyloid, anti-lba1, and anti-SMA.
  • Whole adult Drosophila brain stained with anti-GFP and anti-TH.
  • Whole adult mouse brain stained with anti-TH.
See more

Catalog excerpts

DeepLabel™-1

BEYOND CLEARING, LABELING & IMAGING DeepLabel™ Antibody Staining Kit DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use Without DeepLabel™ reagents optimized for use with clarified tissues. With DeepLabel™, With DeepLabel™ macromolecular probes can rapidly and efficiently penetrate thick, protein-dense tissues for site-specific binding at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. Use DeepLabel™ for vibrant fluorescence imaging at subcellular resolution. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues. FEATURES DeepLabel™ enhances anti-Collegen IV penetration into clarified mouse brain tissues. Lower concentrations of antibodies required Without DeepLabel™ Simple protocol with ready-to-use reagents With DeepLabel™ Multiple rounds of multiplexed antibody labeling 1 mm Antibody labeling ! Tissues cleared with the X-CLARITY™ Systems and Reagents for Tissue Clearing and labeled using the DeepLabel™ Antibody Staining Kit can be imaged with: Light sheet microscopy Two-photon microscopy Confocal microscopy Comparison of antibody staining efficiency with or without DeepLabel™. DeepLabel™ Antibody Staining Kit enhances antibody penetration and site-specific binding in thick, cleared tissues for robust and efficient labeling. Mouse brains labeled with DeepLabel™ had markedly better antibody distribution and low backgr

 Open the catalog to page 1
DeepLabel™-2

OPTIMIZED FOR CLEARED TISSUES DeepLabel™ Solution A C33002 For the permeabilization of clarified tissues to enable the rapid and deep diffusion of macromolecular probes. Non-toxic. DeepLabel™ Solution B C33003 For the enhancement of antibody binding for multiple rounds of multiplexed antibody labeling. Non-toxic. DeepLabel™ Washing Buffer C33004 For washing to remove unbound antibodies and reduce background, thereby increasing signal-to-noise ratio. Non-toxic. X-CLARITY™ Mounting Solution C13101 For the refractive index matching of clarified tissues for optimal imaging. RI = 1.460 at 25°C and...

 Open the catalog to page 2

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.