DeepLabel™
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BEYOND CLEARING, LABELING & IMAGING DeepLabel™ Antibody Staining Kit DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use Without DeepLabel™ reagents optimized for use with clarified tissues. With DeepLabel™, With DeepLabel™ macromolecular probes can rapidly and efficiently penetrate thick, protein-dense tissues for site-specific binding at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. Use DeepLabel™ for vibrant fluorescence imaging at subcellular resolution. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues. FEATURES DeepLabel™ enhances anti-Collegen IV penetration into clarified mouse brain tissues. Lower concentrations of antibodies required Without DeepLabel™ Simple protocol with ready-to-use reagents With DeepLabel™ Multiple rounds of multiplexed antibody labeling 1 mm Antibody labeling ! Tissues cleared with the X-CLARITY™ Systems and Reagents for Tissue Clearing and labeled using the DeepLabel™ Antibody Staining Kit can be imaged with: Light sheet microscopy Two-photon microscopy Confocal microscopy Comparison of antibody staining efficiency with or without DeepLabel™. DeepLabel™ Antibody Staining Kit enhances antibody penetration and site-specific binding in thick, cleared tissues for robust and efficient labeling. Mouse brains labeled with DeepLabel™ had markedly better antibody distribution and low backgr

OPTIMIZED FOR CLEARED TISSUES DeepLabel™ Solution A C33002 For the permeabilization of clarified tissues to enable the rapid and deep diffusion of macromolecular probes. Non-toxic. DeepLabel™ Solution B C33003 For the enhancement of antibody binding for multiple rounds of multiplexed antibody labeling. Non-toxic. DeepLabel™ Washing Buffer C33004 For washing to remove unbound antibodies and reduce background, thereby increasing signal-to-noise ratio. Non-toxic. X-CLARITY™ Mounting Solution C13101 For the refractive index matching of clarified tissues for optimal imaging. RI = 1.460 at 25°C...