SYSTEMS AND REAGENTS FOR TISSUE

SECTIONING IS TIME. WE CUT THE TIME. Tissues are inherently three dimensional in nature, which makes imaging intact tissues a necessity for a more complete study into the relationship between structure and function and the system-level study of cellular mechanisms. Tissue clearing has become an important step for imaging tissues in 3D at single-cell resolution. The X-CLARITY™ is a collection of systems and ready-to-use reagents to standardize, simplify, and accelerate each step of the tissueclearing process. X-CLARITY™ is based on the CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid...

Tissue-hydrogel hybridization X-CLARITY™ Hydrogel Solution Kit The X-CLARITY™ Hydrogel Solution Kit is a pre-tested hydrogel solution for uniform and consistent tissue-hydrogel hybridization. The kit is composed of X-CLARITY™ Hydrogel Solution and X-CLARITY™ Polymerization Initiator . X-CLARITY™ Hydrogel Solution X-CLARITY™ Polymerization Initiator The X-CLARITY™ Hydrogel Solution is a ready- The X-CLARITY™ Polymerization Initiator is a thermal to-use acrylamide-based solution used to create free radical initiator that releases free radicals when polyacrylamide. X-CLARITY™ Hydrogel Solution heated...

Tissue clearing X-CLARITY™ Tissue Clearing System II The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Users can set tissue clearing conditions through a simple and intuitive touchscreen interface. In ETC (electrophoretic tissue clearing) mode, platinum-plated electrodes generate an electric field to accelerate the removal of lipids from tissues in a highly efficient manner. A built-in temperature control system...

Rapid, consistent, and reproducible clearing for thick tissues Long-term preservation of the Thy1-YFP signal in tissues cleared with the X-CLARITY™ systems and reagents. (A) Thy1-YFP signal immediately after clearing. (B) Thy1-YFP signal one month after clearing. Thy1-YFP mouse brain slices cleared with the X-CLARITY™ systems and reagents. Thy1-YFP (green), Anti-Collagen IV (red), TO-PRO-3 (blue). Mouse lungs and trachea cleared with the X-CLARITY™ Mouse heart cleared with the X-CLARITY™ Mouse outer ear cleared with the X-CLARITY™ Mouse liver cleared with the X-CLARITY™ Mouse spinal cord cleared...

Antibody labeling DeepLabel™ Antibody Staining Kit DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all tissue clearing methods including CLARITY, PACT,...

STEP 4 X-CLARITY™ Mounting Solution C13101 - 1 x 25 mL| C13102 - 10 x 25 mL C13107 - 20 x 25 mL X-CLARITY™ Mounting Solution is a refractive index matching solution (RIMS). The RI of the solution is 1.460 at 25°C and is stable over a wide temperature range. X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for mounting clarified and labeled tissue samples. RI = 1.460 at 25°C @ Minimizes photobleaching 0 Preserves fluorescence signals PFA-fixed human cerebral cortex sample cleared with the X-CLARITY™ systems and reagents Courtesy...

Pisa, Italy Paris, France "The [X-CLARITY] has actually been fundamental to the Data Brain Project because without being able to clear tissues ... we wouldn't be able to extract that structural information that is so important to understand the function of mammalian brains. It's been a huge advantage and a great leap forward in imaging." Arti Ahluwalia, PhD Director, Centro E. Piaggio "We purchased the X-CLARITY for our facility and have been very satisfied with its easy-to-use design and consistent results. The X-CLARITY Tissue Clearing System has now allowed many more researchers to enter the...