LoopSeqTM Sequencing
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©Assembly & analysis LoopSeq™ Sequencing The advantages of long-read sequencing. the convenience of your existing sequencing infrastructure Next generation sequencing (NGS) has been an incredibly powerful technique, but there are many applications where short-read sequencing comes up, well, short. Fortunately, Loop Genomics has developed an affordable, easy-to-implement solution that uses existing Illumina NGS sequencers to produce full-length, long-read sequences. Simple, efficient, and powerfully accurate, LoopSeq™ Sequencing Kits and Sequencing Services expand what you can accomplish using NGS. ■ Highly accurate long-read sequencing ■ Leverages your existing short-read sequencing infrastructure ■ Delivers lower error rates than other approaches ■ Uses a simple, one-tube (yes, one tube!) workflow ■ APPLICATIONS: MICROBIOME • TRANSCRIPTOME • GENOME • AMPLICON How does LoopSeq work? It's all in the kit! The LoopSeq workflow is simple, streamlined, and leverages your existing sequencing instrument. OOOOOOOOOOOO OOOOOOOOOOOO OOOOOOOOOOOO OOOOOOOOOOOO OOOOOOOOOOOO OOOOOOOOOOOO OOOOOOOOOOOO ©Long read library preparation Included with every kit

The power of the LoopSeq approach stems from our unique, proprietary barcoding technology and implementation. The key steps of the workflow are described below. Barcoding. Every sample is exposed to millions of unique barcodes, but only one barcode attaches per strand of DNA. Amplification. Every molecule, along with its unique barcode, is amplified using PCR. Distribution. Each copy of the amplified DNA has the barcode randomly distributed to a different location. Sequencing. Sequence the segment next to each barcode. Assembly. Short reads that share the same barcode are combined...