BioLector® Pro Microfluidic Bioprocess Control 32 /48 Parallel Microbioreactors pH Control Continuous Feeding Online Monitoring Scalability Automation

Full Bioprocess Control On-the-Plate BioLector ® Pro The BioLector® Pro is the advanced microbioreactor system combining the established BioLector® technology with an innovative microfluidic chip. The system is based on a standard microtiter plate format and operates with non-invasive, o ptical sensors. The disposable 48 well microtiter plate of the BioLector® Pro features online measurements of biomass, fluorescence, pH and DO and simultaneously controls the pH and f eeding rates through microvalves and microfluidic-channels. This unique microfluidic plate allows continuous feeding and pH control...

Operating Principle Features Online Measurement • Biomass concentration • pH value • Dissolved oxygen (DO) • Riboflavins • Fluorescent molecules (GFP, YFP, DsRed ...) • Temperature • Humidity • O2 in head space atmosphere • CO2 in head space atmosphere Online Control • pH value • Feeding • Shaking speed • Temperature • Humidity • O2 in head space atmosphere • CO2 in head space atmosphere System Performance • Working volume of 800 – 2400 μL • 32 parallel microreactions • 16 reservoir wells • Individual pH control • Continuous individual feeding • Broad range of kLa values • ontinuous gas exchange...

Advantages • Real-time kinetics out of 32 parallel fermentations • Microfermentation in standard MTP format • Batch and fed-batch cultivation • Control of pH on-the-plate • Continuous controlled feeding on-the-plate • DO- and signal-triggered feeding • Low pH measurements in the range of 4-6 • High-throughput and easy automation • road range for biomass detection (equivalent to B up to 250 OD600, 50 g/L CDW, measured with E. coli ) • iomass measurement is online and does B not require dilution • Small volume (800 – 2400 µL) • No edge effects • Continuous shaking operation (no artifacts) • Defined...

System Art.-No.: G-BLMF 100Note: The BioLector® Pro includes the BioLection software. Operation conditions Plate format Filter configuration Ranges, measurement and pH control Calibration Precalibrated plates control range pH ~4 - 6 (low pH module) with < 0.1 deviation Ranges are broader with less accuracy Measurement range DO 0 - 100 % oxygen saturation pH control By acid or/and base Application mode Disposable technology Rated power 280 W BioLector® Pro, 120 W add. valve control unit Interface Ethernet Automation Optionally, the BioLector® can be integrated into the robotic liquid handling...

m2p-labs is an internationally leading supplier of microbioreactors. The company focuses on microreaction and automated solutions for screening and bioprocess development. The microfermentation technology enables customers to conduct experiments with great efficiency and excellent quality at low costs. More knowledge from small scale leads to more rational and reliable decisions in the development of bioprocesses. EUROPE m2p-labs GmbH Arnold-Sommerfeld-Ring 2 52499 Baesweiler Germany Phone +49-2401-805-330 Fax +49-2401-805-333 [email protected] USA m2p-labs, Inc. 62-64 Enter Lane Islandia, NY...