Datasheet & Protocol ELISA Flex kit for quantitative determination of native and recombinant porcine IL-17A in solution, e.g. cell supernatant. The kit includes for 6 plates for 20 plates To ensure total recovery of the stated quantity, vials have been overfilled. Shipping and storage Shipped at ambient temperature. All reagents should be stored at 4-8 °C upon receipt, except the standard which should be stored at -20 °C. Antibodies are supplied in sterile-filtered PBS with sodium azide (0.02%). Streptavidin-ALP is supplied in 0.1 M Tris buffer with 0.002% Kathon CG. The expiry date indicates how long unopened products, stored according to instructions, are recommended for use.

Specificity The kit contains a matched pair of monoclonal antibodies (mAbs) enabling specific detection of porcine IL-17A. The ELISA standard is recombinant bovine IL-17A. Standard range 10-1000 pg/ml Calibration The standard has been calibrated to recombinant porcine IL-17A. No international standard exists for calibration. Reconstitution of ELISA standard Reconstitute the ELISA standard to a stock solution of 0.05 pg/ml by adding 1 ml of the standard reconstitution buffer. Allow the standard to dissolve for 5 minutes and mix thoroughly. The standard should be kept in aliquots at -20 °C. Avoid...

Add 100 μl/well of capture mAb MT49A7 diluted to 2 μg/ml in PBS, pH 7.4. Use high protein binding ELISA plates. Incubate overnight at 4-8 °C. Empty the plate and add 200 μl/well of PBS with 0.05% Tween 20 and 0.1% BSA (incubation buffer) to block the plate. Incubate for 1 hour at room temperature. Wash the plate 5 times with PBS containing 0.05% Tween 20 (300 μl/well). Add 100 μl/well of samples or standards diluted in incubation buffer. Include assay background control, i.e. wells without standard. Incubate for 2 hours at room temperature. Add 100 μl/well of detection mAb MTP853-biotin diluted...

Updated on 2023/03/24 Developed and manufactured by MABTECH AB, Sweden, whose quality management system complies with the standards ISO 9001:2015 & ISO 13485:2016. Quality management systems Medical devices Quality management systems The products are for research use only. MABTECH shall not be liable for the use or handling of the product or for consequential, special, indirect or incidental damages there from. Mabtech AB (Head Office) Sweden Tel: +46 8 716 27 00 [email protected]