CONTENTS: ► Capture mAbs: Anti-Ig k A mAbs (MT24/JC5-1) 800 |il Concentration: 0.5 mg/ml ► Detection mAb: Anti-IgM mAb (MT9A2), 490 25 |il ► Detection mAb: Anti-IgA mAb (MT39A), 640 25 |il ► Polyclonal activators: R848, 100 |il Lyophilized recombinant mouse IL-2, 0.5 ^g ► FluoroSpot enhancer, 25 ml ► FluoroSpot plate (1 IPFL plate) Capture antibodies are supplied in sterile filtered (0.2 ^m) PBS with 0.02% sodium azide. R848 is supplied in sterile filtered (0.2 ^m) PBS with 2% DMSO. Fluorophore conjugated detection antibodies and FluoroSpot enhancer contain 0.002% Kathon CG. STORAGE: Shipped at ambient temperature. Store antibodies and FluoroSpot enhancer at 4-8°C upon reciept. R848 and IL-2 should be stored at -20°C. Plates may be kept at room temperature.

Guidelines for Mouse IgM/IgA FluoroSpot This kit contains reagents for the detection of mouse IgM and IgA secreting B cells. B cells secreting antigen-specific antibodies as well as B cells secreting antibodies irrespective of antigen specificity can be measured with this kit. For analysis of memory B cells, cells may need to be pre-activated (see Hints and comments). Antigen-specific IgM/IgA FluoroSpot: Antigen is coated on the FluoroSpot plate and antigen-specific IgM and IgA secreted by B cells bind to the immobilized antigen. Spots are visualized after addition of fluorophore labeled antibodies....

Hints and comments These suggestions are based on the detection of antigen-specific immune responses using spleen cells. If using B-cell clones, mixtures of separated cell fractions etc., other protocols may have to be considered. Plates The IPFL plates included in the kit have a low fluorescent PVDF-based membrane. To obtain maximal antibody binding capacity the plates need to first be activated by a brief treatment with ethanol. It is essential that the membrane is not allowed to dry after the treatment. If this occurs the treatment step (A2-3) needs to be repeated before adding the coating...

Updated on 2023-02-02 Developed and manufactured by MABTECH AB, Sweden, whose quality management system complies with the standards ISO 9001:2015 & ISO 13485:2016. The products are for research use only. Quality management systems Medical devices Quality management systems MABTECH shall not be liable for the use or handling of the product or for consequential, special, indirect or incidental damages there from. Mabtech AB (Head Office)