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MagSi-cfDNA

Product catalog summary

Overview: The MagSi-cfDNA kit is designed for the automated purification of circulating cell-free DNA (cfDNA) from human plasma or serum. It is optimized for use with the PurePrep 24 system and can process 24 samples in approximately 60 minutes without the need for phenol/chloroform extraction or alcohol precipitation.

Features & Benefits:

Compatible with fresh or frozen plasma or serum samples collected in various blood collection tubes (e.g., Streck Cell-Free DNA BCT, EDTA, Citrate).
Reagents provided for 96 cfDNA extractions from 2 mL samples, scalable for 1 to 4 mL, with support for 10 mL samples.
Typical cfDNA yield ranges from 0.5 to 4 ng per mL of plasma, though this is donor-dependent.
Post-lysis steps are performed at room temperature, and no carrier RNA is required.
Magnetic beads are supplied in a buffer that reduces sedimentation time.
Suitable for downstream applications such as RT-qPCR and sequencing.

Performance Data:

Figures demonstrate high cfDNA yields from serum and Streck plasma, with scalable results.
Comparison with competitor kits shows higher cfDNA yields and purity with the MagSi-cfDNA kit.
Electropherograms indicate high integrity and quality of cfDNA, with minimal contamination.

Ordering Information: The kit is available under Art. No. MDKT00220096 for 96 preparations. For more information, contact magtivio B.V. at [email protected] or visit their website.

Catalog excerpts

Purification of circulating cell-free DNA from human plasma or serum

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Purification of circulating cell-free DNA from human plasma or serum MagSi-cfDNA is intended for (automated) purification of circulating cell-free DNA from human plasma or serum samples. Processing time for the automated preparation of 24 samples is about 60 minutes. The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples. The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.

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