MagSi-NGSPREP Plus

Product catalog summary

Overview
MagSi-NGSPREP Plus is a magnetic bead-based tool designed for size selection and clean-up in Next-Generation Sequencing (NGS) library preparation. It offers a convenient and efficient method for DNA purification and size selection, optimized for automation on various laboratory workstations.

Specifications

Compatible with Biomek® Laboratory Automation Workstations and Hamilton® Microlab STARline.
Works with multiple automated liquid handling systems, including PerkinElmer® and Agilent Technologies®.
Available in volumes of 5 mL, 75 mL, and 500 mL.
Utilizes 96 and 384 well plate magnetic separators for optimized separation performance.

Procedures

Follows a simple bind-wash-elute procedure with a short process time.
Allows for non-selective or size-targeted binding of DNA fragments ranging from 80 to 1000 base pairs.
Adjustable reagent-to-sample ratios for specific clean-up or size selection needs.

Performance and Results

Based on proven SPRI® technology for high recovery and purity of PCR products.
Ensures excellent removal of contaminants such as enzymes, primers, and salts.
Fragment size selection can be tuned between 100 and 1000 base pairs, guaranteeing consistent sequencing results.

Automation and Compatibility

Easy to automate for high-throughput processing.
Compatible with standard protocols of common library preparation kits.
Manual and automated use supported.

Figures and Data

Figures illustrate size selection fraction retained by beads with different reagent-to-sample volume ratios and magnetic separators for automated processing.
DNA size distribution curves demonstrate a direct comparison between MagSi-NGSPREP Plus and AMPure® XP using different bead-to-sample ratios.

Catalog excerpts

MagSi-NGSPREP Plus Size selection and clean-up in NGS library preparation

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Magnetic bead-based MagSi-NGSPREP Plus provides a convenient tool for ultra-fast and efficient purification and size selection of DNA products. The kit is optimized for use on Biomek® Laboratory Automation workstations and Hamilton® Microlab STARline. MagSi-NGSPREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 - 1000 bp with specific reagent volume to sample volume ratio's. By increasing the volume of MagSi-NGSPREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard...

Open the catalog to page 2

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