MagSi-STA Trial kit
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Product Description Art.No.: MD50001 magtivio I. Intended Use MagSi-STA beads are suitable for numerous In Vitro Diagnostics (IVD) applications, including immunoassays. The MagSi-STA Trial kit is intended for evaluation of different MagSi-STA beads for assay development, and includes MagSi-STA beads with various physical and chemical properties in order to find the best suitable bead for your specific application. II. Principle MagSi-STA beads are added to a sample containing biotinylated molecules. The biotinylated molecules will bind to the beads during a short incubation. The complex is separated from the sample using a magnet and can be used analyte capture. Binding of molecules onto MagSi-STA is based on the strong non-covalent interaction between streptavidin and biotin (Ka = 1015 M-1). Biotin is easily conjugated to various molecules and inexpensive biotinylated products are sold by many companies. MagSi-STA beads are coated with recombinant streptavidin (53 kDa) with shortened N- and C-terminus for improved solubility and accessibility of the sites. Albumin binding sites are eliminated for optimal specificity. All MagSi beads included in this kit consist of a silica matrix, which does not have luminescent properties like for instance polystyrene. This results in comparatively low background signals in chemiluminescence- and UV/VIS readout and enhances the assay sensitivity and dynamic range. Therefore, MagSi-STA beads are suitable for any assay, regardless of read out method used. The silica matrix ensures product stability over a wide pH range and at high ionic strength conditions. magtivio's production process has been developed for a very flexible but controlled magnetic bead synthesis and streptavidin coating. This process enables magtivio to set the range for biotin binding capacity as narrow as needed (±10%). For some application this is important because the parameters in play, like bead concentration, total amount of beads and binding kinetics of biotinylated targets, can be set as fixed values in optimizing and validating an IVD assay. The streptavidin surface coating can be controlled with low variation for a broad range of desired binding capacities (up to 8000 pmol free biotin/mg beads, with ±10%) to ensure optimal reproducibility for your applications. III. Material Supplied Table 1: MagSi-STA beads supplied in this MagSi-STA Trial kit MagSi-STA beads are supplied as a suspension containing 10 mg magnetic beads per mL, in PBS (pH 7.4). The products contain 0.05% Tween20 and 0.05% sodium azide as preservative. The MagSi-STA Trial kit contains 8 x 1 mL of each MagSi-STA product as described in Table 1. Bead Size MagSi-STA beads are available in sizes of 600 nm, 1.0 pm and 3.0 pm. The sedimentation time of 600 nm beads has been optimized and is approx. 4 times compared to 1.0 pm beads. This allows e.g. long incubation times without shaking/mixing etc. MagSi-STA beads with a diameter of 3.0 pm have stronger magnetic properties and will separate approx. 3x faster compared to 1.0 pm under the same conditions. Surface Activation Two types of surface activation for streptavidin coating are included in our kit . Carboxyl-activation is used for higher binding capacities of MagSi-STA beads. Tosyl-activation is typically used for lower binding capacities. Tosyl-activated beads are also more hydrophobic than carboxyl-activated beads. Because of differences in hydrophobicity, the type of surface activation may also influence the sensitivity of the assay. Binding Capacity The optimal binding capacity of streptavidin coated magnetic beads depends on the sensitivity requirements of an assay. In this kit, beads with different binding capacities are included, covering the total range of free biotin binding capacities of competitor beads currently used in the market, and even more. It is intended to find the bead with the optimal binding capacity for your specific application.

Product Description Art.No.: MD50001 magtivio IV. Product Use When stored at 2-8°C, this product is stable for up to 2 years, but no longer than the expiry date on the label (for bulk deliveries of MagSi-STA products, a shelf-life of 3 years is guaranteed). Store in well closed vial and in upright position to prevent drying of the beads, because this may result in a decrease of activity. Do not freeze the product! Vortex well before use. Wash the beads to remove preservatives that could interfere with your application. For washing, use the same volume as initially taken from the MagSi-STA...