Malvern MicroCal Differential Scanning Calorimetry (DSC) Systems
14Pages

LABEL-FREE BINDING ANALYSIS MICROCALORIMETRY LABEL-FREE BINDING ANALYSIS MICROCALORIMETRY MICROCAL DSC SYSTEMS UNDERSTANDING BIOMOLECULAR STABILITY

Understanding biomolecular interactions CHARACTERIZE BIOMOLECULE STABILITY QUICKLY WITHOUT LABELS OR PROBES Differential Scanning Calorimetry (DSC) is a powerful analytical tool for characterizing the stability of proteins and other biomolecules. DSC directly measures the enthalpy (ΔH) and temperature (Tm) of thermally induced structural transitions in solution. This information can be used to predict shelf lives, develop purification strategies, and characterize and evaluate protein constructs or other biotherapeutic entities. In addition, it permits rapid ranking of ligand affinities to a...

Understanding biomolecular interactions KEY BENEFITS OF MICROCAL DSC SYSTEMS MicroCal DSC differential scanning microcalorimeters provide fast and accurate determination of transition midpoint (Tm) and other thermodynamic parameters as indicators of thermal stability. They generate a complete thermodynamic profile to understand the factors that affect a biomolecule’s conformation and stability. A choice of systems meets the requirements of different laboratories: • MicroCal VP-Capillary DSC delivers high throughput and sensitivity, with low sample consumption, in an automated, integrated...

Understanding biomolecular interactions INTRODUCTION TO DIFFERENTIAL SCANNING CALORIMETRY Differential Scanning Calorimetry (DSC) measures heat changes associated with the thermal denaturation of proteins and other biomolecules, and is used to characterize their stability. A biomolecule in solution is in equilibrium between the native (folded) conformation and its denatured (unfolded) state. DSC measures the heat absorbed when the molecule undergoes ‘melting’ from its native, biologically active conformation to an unstructured, inactive conformation. The measured thermal transition midpoint...

Understanding biomolecular interactions Theory into practice Figure 3. 50 Studying the Tm shift over a range of different ligand concentrations provides data that can be used to calculate the affinity of the interaction. An increase in Tm is associated with an increase in stability. Stabilizing or destabilizing events arise from two different sources: extrinsic and intrinsic. Intrinsic factors are events that occur within the molecule, such as truncation of the molecule, mutation or the addition of an R-group. Extrinsic factors are those that are external to a molecule. Solution conditions...

Understanding biomolecular interactions DSC IN ACTION - DEVELOPING STABLE BIOTHERAPEUTICS Maintaining active, safe and effective biotherapeutics, from development and manufacturing through to targeted delivery in the patient, is a key industry challenge. DSC is a proven and widely-used stability indicating technique. MicroCal DSC systems are integrated into the workflow of pharmaceutical and biotechnology industries, academia and government institutions worldwide. While the following examples provide a snapshot of their use, you can find a wealth of applications information at:...

Understanding biomolecular interactions Process development - optimization of purification conditions 1. Biotherapeutic downstream production processes must achieve high purity and adequate yield on a large scale and within a given economic framework. Using DSC, the most stabilizing loading and elution conditions can be established during process development. In Figure 5, DSC is used to establish the stability of an antibody in a range of buffers. The antibodies bind at around pH 7 and are eluted at a lower pH. Maintaining antibody stability during elution can be a challenge so identifying...

Understanding biomolecular interactions MALVERN MICROCAL DSC AT A GLANCE MicroCal VP-Capillary DSC The VP-Capillary DSC System is a highly sensitive differential scanning microcalorimeter for characterizing the stability of proteins and other biomolecules. It has an active cell volume of 130 µL, allowing thermodynamic measurements of even precious samples and typically requiring sample concentrations of around 0.2 mg/mL. For high throughput work, a fully integrated autosampler enables running of up to 50 samples per day. All filling, injection, and cell cleaning functions are fully...

Understanding biomolecular interactions MicroCal VP-DSC The MicroCal VP-DSC is a highly sensitive, easy-to-use differential scanning microcalorimeter for the study of samples in solution. Providing fast, accurate transition midpoint (Tm) determination, the MicroCal VP-DSC revolutionized the study of liquid biopharmaceutical formulations by reducing the time and cost of stability testing. In addition, a complete thermodynamic profile is generated to understand the factors that affect conformation and stability. The VP-DSC is controlled by an intelligent user interface (VPViewer software) and...

Understanding biomolecular interactions MicroCal VP-Capillary DSC Software brings a new level of productivity to automated DSC. Designed to streamline workflow by providing simplified experimental set up it ncludes, for example, an Excel sample list template that can be saved and re-used rather than rewritten The software enables flexible instrument scheduling that is ideal for the multi-user laboratory. In addition, data analysis has been automated and baselines and buffer scans are automatically detected and subtracted, for straightforward analysis Key parameters such as Tm and Tm onset...

Understanding biomolecular interactions ] Rnase only - no ligand

Understanding biomolecular interactions SPECIFICATION COMPARISON SUMMARY

Malvern’s materials characterization technology and expertise enables scientists and engineers to understand and control properties of dispersed systems. Malvern’s instruments are used to measure particle size, particle shape, zeta potential, molecular weight, size and conformation, rheology and for chemical identification. This information helps accelerate R&D, enhance product quality, optimize process efficiency. Areas we work in: • ACADEMIC BIOCHEMICAL RESEARCH • BIOPHARMACEUTICALS • FOOD AND DRINK • ASPHALT • PHARMACEUTICAL • COSMETICS AND PERSONAL CARE • CHEMICALS • MINING AND MINERALS...